Molecular Identification, Isolation and Evaluation of Persian Gulf Actinomycetes as Candidates of Cytotoxic Metabolites Against Breast Cancer
Introduction: Marine actinomycetes have a great potential to produce unique bioactive compounds due to their special adaptation in the harsh ocean environment. The current study aimed to isolate anti-cancer compounds producing actinomycetes from sediments of Harra forests of the Persian Gulf and investigate their potential as anti-breast cancer metabolites. Methods: In the current study, 40 sediment samples of Harra forests of the Persian Gulf were collected. Samples were diluted and cultured in a starch casein agar selective medium. The strains were isolated and purified, using morphological and microscopic methods. Forty strains were cultured in a starch casein broth and the metabolites were extracted using ethyl acetate. The produced metabolites were extracted from active strains and their cytocidal activities were evaluated against the breast cancer cell line. Finally, effective-metabolites-producing bacteria were identified using the molecular method. Results: Of the sediments, 186 strains were isolated and identified. Results showed that the isolates had cytocidal activities against the breast cancer cell line. The results also revealed that the 2HP and 4HP strains showed more anti-cancer activities than the others. Results of this study showed that sediments of Harra forests of the Persian Gulf were rich in active actinomycetes, which can be used in the production of new anticancer compounds. Conclusions: The obtained results give evidence that it is essential to scrutinize these marine microorganisms, which have a great potential to be used in the pharmaceutical fields, in search for new drugs.
Isolation and evaluation of cytotoxic and antioxidant activity of bioactive metabolites of cultured actinobacteria in Persian Gulf marine sediments
Screening bacteria for bioactive compounds is the first step in the drug discovery programs. The aim of the present research was to achieve cytotoxic and antioxidant metabolites from actinobacteria of Persian Gulf sediments and to investigate the effects of combinations of seven treatments and six isolation media on the selective isolation process. A total of 395 actinobacterial isolates were c...متن کامل
Introduction: L-asparaginase is an anti-neoblastic agent used in the chemotherapy of lymphoblastic leukemia. This enzyme is widely distributed among microorganisms, plants and animals, but microorganisms have proved to be a better alternative for L-asparaginase, thus facilitating its large-scale production. Actinomycetes are filamentous, Gram-positive bacteria widely distributed in the marine e...متن کامل
investigating the cytotoxic effects of persian gulf marine actinomycetes protease on blood cancer cell line
background: considering their specific adaption to marine milieus, marine actinomycetes constitute an excellent source for producing unique bioactive substances. they are also important sources of antibiotics and extracellular enzymes, geosmin, and enzymes with potent antibacterial and anticarcinogenic properties and low toxicity. the objective of this study is to isolate actinomycetes producin...متن کامل
Antibacterial activity of actinomycetes isolated from native sponge of Persian Gulf (Kharku Island) against pathogens
Resistant of pathogens to common antibiotics is a major global problem, and efforts discover new antibiotics from less known resources, is an inevitable. Native sponges of Persian Gulf have high potential in the field of medical biology, but have received little attention. In this study, actinomycetes that capable to produce anti-bacterial metabolites isolated and examined. Two sponges of Persi...متن کامل
the study of aaag repeat polymorphism in promoter of errg gene and its association with the risk of breast cancer in isfahan region
چکیده: سرطان پستان دومین عامل مرگ مرتبط با سرطان در خانم ها است. از آنجا که سرطان پستان یک تومور وابسته به هورمون است، می تواند توسط وضعیت هورمون های استروئیدی شامل استروژن و پروژسترون تنظیم شود. استروژن نقش مهمی در توسعه و پیشرفت سرطان پستان ایفا می کند و تاثیر خود را روی بیان ژن های هدف از طریق گیرنده های استروژن اعمال می کند. اما گروه دیگری از گیرنده های هسته ای به نام گیرنده های مرتبط به ا...15 صفحه اول
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دوره 1 شماره None
صفحات 10- 14
تاریخ انتشار 2017-07
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