Time dependent difference effects of MgO and ZnO nanoparticles on the serum and hippocampus Mg2+, Zn2+, Fe2+/3+ and Ca2+ levels in the stressed rats

Objective(s): Stress is a physiological response that can disrupt body elements homeostasis and lead to neurophysiological abnormality. This study has been investigated the serum and hippocampus Mg2+, Zn2+, Fe2+/3+ and Ca2+ level changes in two times after MgO NPs and ZnO NPs single injection following restraint stress in the male rat. Methods: Animals were divided into two main groups that each of them includes: control, restraint of 90, 180 and 360 min+ saline, MgO NPs and ZnO NPs 5 mg/kg alone and with a restraint of 90 min. In one group, 30 min and in another 120 min after intraperitoneally injections of components or stress induction elements levels were measured in the serum and hippocampus. Results: MgO NPs and ZnO NPs could change elements level in the serum and hippocampus depend on acute time after injections and there was a significant positive correlation between serums Fe2+/3+ following two different acute times after ZnO NPs administration. Different times of stress induction have different effects on elements level changes in the serum and hippocampus, 30 and 120 min after induction and nanoparticles could alleviate these changes depend on the time. In restraint groups, there were positive and negative significant correlations between two different times measurements of Fe2+/3+ or Ca2+ in the serum and hippocampus. Conclusion: it seems that time is an important factor in ameliorative MgO NPs and ZnO NPs effects on elements disruption induced by stress, but their exact interaction with stress systems containing ions level changes needs to more investigation.