Two Steps Methylation Specific PCR for Assessment of APC Promoter Methylation in Gastric Adenocarcinoma
Gastric Cancer (GC) is the second most common cancer in the world and a leading cause of cancer-related mortality. Methylation of promoter CpG islands (CGIs) belonging to tumor suppressor genes causes transcriptional silencing of their corresponding genes leading to carcinogenesis and other disorders. Adenomatous Polyposis Coli (APC) a tumor suppressor gene is inactivated by methylation of promoter region in CpG islands. In current study, methylation of CGIs in the promoter of APC was analyzed using two steps Methylation Specific PCR in 36 tumor tissue samples together with an equal number of normal tissues belonging to patients with confirmed diagnosis of gastric carcinoma. Methylation specific PCR (MSP) was positive for 28 out of 36 (77.77%) of the tumors. No correlation was found between APC hypermethylation and the age of patients regardless of the relevant cofactors in aberrant methylation. Our study showed that hypermethylation of the APC gene was frequent among our patients with gastric cancer. Although the APC hypermethylation has been proposed to be involved in the early stages of gastric carcinogenesis, but the APC gene alone, does not seem to be a reliable marker for the different stages of cancer prognosis. Thus, it is recommended to use a panel of genes for evaluating the CpG Island methylator phenotype (CIMP) phenomena as an early event in gastric cancer, both for tumor and serum samples.
two steps methylation specific pcr for assessment of apc promoter methylation in gastric adenocarcinoma
gastric cancer (gc) is the second most common cancer in the world and a leading cause of cancer-related mortality. methylation of promoter cpg islands (cgis) belonging to tumor suppressor genes causes transcriptional silencing of their corresponding genes leading to carcinogenesis and other disorders. adenomatous polyposis coli (apc) a tumor suppressor gene is inactivated by methylation of prom...متن کامل
Direct Bisulfite Sequencing and Methylation Specific PCR to Detect Methylation of p15INK4b and F7 genes in Coronary Artery Disease Patients
Genome-Wide Association Studies (GWAS) have identified genetic variants contributing to the risk of cardiovascular disease (CVD) at the chromosome 9p21 locus. The chromosome 9p21 is an important susceptibility locus for several multifactorial diseases like ischemic stroke, aortic aneurysm, type 2 diabetes mellitus and coronary artery disease (CAD). F7 gene because of its role in activating the ...متن کامل
In recent years, several methylation-specific PCR-based techniques have been developed to identify and characterize hypermethylation of CpG dinucleotides with the primary goal of elucidating a better understanding of the role of DNA methylation in important biological processes, such as chromosome X inactivation and carcinogenesis. The specificity of methylation-specific PCR (MSP) techniques re...متن کامل
Performance of methylation and expression fluctuations of sonic hedgehog genes in gastric adenocarcinoma
Gastric cancer (GC) is considered as one of the most serious cancers, and in Iran, due to environmental factors in different regions, it has a high frequency. Apart from environmental factors, genetic and epigenetic ones also play a key role in the development of carcinogenesis of GC. In this regard, the study of functioning of the molecular mechanisms involved in the carcinogenesis and also tu...متن کامل
Analyses of methylation status of CpG islands in promoters of miR-9 genes family in human gastric adenocarcinoma
In the recent years deregulation for microRNAs expression pattern have emerged as a possible molecular factor for carcinogenesis. It has been reported that the expression of miR-9 was down-regulated in human gastric adenocarcinoma. To figure out the molecular mechanism of this down regulation, the methylation status in promoters of miR-9 family loci were compared in the human gastric adenocarci...متن کامل
Methylation of the DNA is an important epigenetic (i.e., not associated with alteration in the primary structure of the DNA) phenomenon, which plays important roles in regulation of gene expression, maintenance of genome integrity, and genomic imprinting. Although not only other nucleotides, but also proteins and lipids can be methylated, in the context of the present discussion, “methylation” ...متن کامل
دوره 22 شماره 3
صفحات 205- 212
تاریخ انتشار 2011-09-01
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