Kinesin family member C1 (KIFC1) is the only member of the minus-end-directed kinesin-14 family in human cells. In cancer cells, KIFC1 plays an essential role in bipolar spindle formation by clustering the multiple poles during mitosis. However, it has not been clearly demonstrated whether KIFC1 also functions to mediate bipolar spindle formation and to maintain genomic stability in normal cell...

OBJECTIVES This study sought to investigate a novel method to calculate the index of microcirculatory resistance (IMR) in the presence of significant epicardial stenosis without the need for balloon dilation to measure the coronary wedge pressure (P(w)). BACKGROUND The IMR provides a quantitative measure of coronary microvasculature status. However, in the presence of significant epicardial s...

Shrimp white spot disease (WSD), which is caused by white spot syndrome virus (WSSV), is one of the world's most serious shrimp diseases. Our objective in this study was to use an immunomagnetic reduction (IMR) assay to develop a highly sensitive, automatic WSSV detection platform targeted against ICP11 (the most highly expressed WSSV protein). After characterizing the magnetic reagents (Fe3O4 ...

BACKGROUND Magnetic nanoparticles biofunctionalized with antibodies are able to recognize and bind to the corresponding antigens. In this work, anti-C-reactive protein (CRP) antibody was covalently conjugated onto the surface of magnetic nanoparticles to label CRP specifically in serum. METHODS The level of serum CRP was detected by immunomagnetic reduction (IMR) assay, which identifies the c...

BACKGROUND The pathophysiology of myocardial injury and repair in patients with ST-elevation myocardial infarction is incompletely understood. We investigated the relationships among culprit artery microvascular resistance, myocardial salvage, and ventricular function. METHODS AND RESULTS The index of microvascular resistance (IMR) was measured by means of a pressure- and temperature-sensitiv...

VOLUME 89, NO. 3 Infant mortality is a frequently used measure of population health status. Because of concern that Canada’s infant mortality rate (IMR) was rising, Joseph and Kramer examined trends in Canada’s IMR and the effect of registering extremely low birthweight (under 500 grams) infants as live births. A live birth is defined as any infant who, after delivery, “...breathes or shows any...

We developed an anti-gene peptide nucleic acid (PNA) for selective inhibition of MYCN transcription in neuroblastoma cells, targeted against a unique sequence in the antisense DNA strand of exon 2 of MYCN and linked at its NH(2) terminus to a nuclear localization signal peptide. Fluorescence microscopy showed specific nuclear delivery of the PNA in six human neuroblastoma cell lines: GI-LI-N an...

OBJECTIVE To morphometrically compare local atherosclerotic changes in cancerous prostate with those in noncancerous prostate specimens, as epidemiological studies report a positive association between the prevalence of general atherosclerosis and prostate cancer. MATERIALS AND METHODS The intima-to-media-ratio (IMR) of 538 prostate capsular tissue arteries was measured in 50 prostate cancer-...

AIM To assess whether secular trends in stomach cancer mortality were correlated with trends in infant mortality rate (IMR) or gross domestic product (GDP). METHODS Data from seven European countries were analyzed. We used Poisson regression to describe mortality trends among birth cohorts of 1865-1939 and correlation coefficients to determine associations with IMR/GDP. RESULTS Large differ...

OBJECTIVE Utilising a novel study design, we evaluated serial measurements of the index of microcirculatory resistance (IMR) in patients undergoing primary percutaneous coronary intervention (PPCI) for ST-segment elevation myocardial infarction (STEMI) to assess the impact of device therapy on microvascular function, and determine what proportion of microvascular injury is related to the PPCI p...