BACKGROUND It is necessary to develop a highly specific and sensitive assay to quantify the exact amount of hepatitis C virus (HCV) RNA in blood of patients with hepatitis C. For this reason, a real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay for quantification of HCV RNA in human plasma was developed. METHODS A pair of primers as well as hybridization probes were sele...

BACKGROUND Successful treatment to eliminate HCV RNA depends on the identified genotype. In the present study, we compared the frequency of different HCV genotypes, during four years study (2004 till 2008). METHODS Sera specimens were received from 16 provinces of Iran. We used High Pure Viral Nucleic Acid Purification kit for extraction and samples were tested with improved form of RT-PCR te...

Background Assays that are used for the diagnosis and management of HIV-1 infection are subject to assay interference(s) and co-infection with HCV may interfere with HIV-1 genotyping and/or phenotyping. Since a third of HIV-1 infected patients are frequently HCV co-infected, there is an increasing need to assess the effect of HCV coinfection on the accuracy of the HIV-1 protease (PR) and revers...

BACKGROUND AND AIMS Despite the discovery of hepatitis C virus (HCV) entry factor, the mechanism by which it is regulated by miRNAs remains unclear. Adipose tissue-derived human mesenchymal stem cells (AT-hMSCs) have been widely used for differentiated hepatocyte-like cells (DHCs). Here, we established an in vitro HCV infection model using DHCs from AT-hMSCs and identified miRNAs that modulate ...

BACKGROUND Hepatitis C virus (HCV) infection is highly prevalent in renal transplant (RT) recipients. Currently, interferon-based (IFN-based) antiviral therapies are the standard approach to control HCV infection. In a post-transplantation setting, however, IFN-based therapies appear to have limited efficacy and their use remains controversial. The present study aimed to evaluate the efficacy a...

BACKGROUND Distribution of Hepatitis C Virus (HCV) genotypes may be changed over time. Epidemiological Studies on distribution patterns of HCV genotypes in Pakistani population might assist for better treatment options and preventive strategies. OBJECTIVES This study was conducted to determine distribution patterns of HCV genotypes in different geographical regions of Pakistan. PATIENTS AND...

objective: in this project, our aim was to construct a novel expressing vector harboring a new sequence from overlapping region of ns3 gene of hcv from infected iranian patient. materials and methods: the partial ns3 (pns3) gene was amplified by nested-rt-pcr method using sera of hcv infected patients harboring genotype 1a. after purification and cloning the pns3 into ta-cloning vector, the be...

conclusions the prevalence of hbv infection among donors in duhok was comparable with those of reports from other parts of iraq. education and immunization should be initiated to target the high-risk groups. furthermore, a community-based study is recommended to study the prevalence of hbv and hcv infection in the population. background the prevalence of blood borne viral infection is escalatin...

BACKGROUND AND OBJECTIVES Hepatitis C virus (HCV) is a major public health problem worldwide. Replication and persistence of HCV genome have been described in the liver tissue as well as B cells lymphocyte. Several investigations have reported that long-term persistence of HCV in B cells may result in Hodgkin and Non-Hodgkin lymphoma. This study was aimed to determine frequency of HCV RNA in hi...