Immunoblotting studies with antisera against Z-protein, desmin, and alpha-actinin showed that Z-protein is clearly distinguishable from desmin and alpha-actinin. Z-protein is not a proteolytic product of another protein but is an intrinsic component of chicken breast muscle myofibrils. In these experiments, an SDS extract of intact muscle was first electrophoresed in a polyacrylamide gel, and t...

The present study was conducted to investigate the effect of immunoneutralization against endogenous inhibin on oocyte and embryo production in adult and immature mice. At 12:00 h on day 2 of oestrus (day 1 of dioestrus), a single i.p. injection of inhibin antiserum (50, 100, 200 or 400 microl per animal) or equine chorionic gonadotrophin (eCG; 10 or 20 iu per animal) or control goat serum (100...

Yeast and hyphal walls of Candida albicans were extracted with sodium dodecyl sulfate (SDS). Some of the extracted proteins reacted with a specific beta-1,6-glucan antiserum but not with a beta-1,3-glucan antiserum. They lost their beta-1,6-glucan epitope after treatment with ice-cold aqueous hydrofluoric acid, suggesting that beta-1,6-glucan was linked to the protein through a phosphodiester b...

The measurement of immunoglobulin concentrations in serum by a turbidimetric method using a centrifugal analyser (Cobas Bio) is described. In the procedure described it is possible to measure the absorbance of each sample in the buffer reagent in a preliminary run without antiserum (sample blank for correction of colour, turbidity, etc.) In the main run (after adding antiserum) a final measurem...

Prolactin administration reportedly increases blood pressure in rats and rabbits. To study the effects of prolactin deficiency on blood pressure, rats were given saline, normal rabbit serum, or rabbit antiserum to rat prolactin on postnatal days 2 to 5. Both males and females given antiserum had significantly lower blood pressure at 14 weeks than rats given saline or normal rabbit serum. Blood ...

An antiserumto humanmelanomahasbeenproducedin a chimpanzee by hyperimmunization with melanoma cells from a single donor. After absorption with the peripheral blood lymphocytes of the tumor donor, this antiserum is specifically cytotoxic to melanoma cells from 14 tissue culture cell lines and to cells from 8 fetal fibroblast cell lines. Peripheral blood lymphocyte-absorbed antiserum is nega tive...

Cholera enterotoxin (CT) and heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli share a common antigenic determinant. In addition, CT and LT each have a unique antigenic determinant. Antisera were prepared by immunoaffinity chromatography against these unique antigenic determinants, that is, antiserum that reacted with CT but not with LT and antiserum that reacted with LT...

Immunologic cross-reactivity between Streptococcus pyogenes type A exotoxin (erythrogenic toxin) and Staphylococcus aureus enterotoxins B and C1 was demonstrated by Ouchterlony double diffusion, Western immunoblot, and immunodot analyses. Specific antiserum to type A streptococcal exotoxin reacted more strongly with staphylococcal enterotoxin B than with enterotoxin C1. The reactivity of type A...

Factitious proteinuria is an unusual finding. We present a case in which clinical suspicion was aroused by the disparity between the clinical history and findings and the 24-h excretion of protein in urine. Electrophoresis of the patient's serum and urine confirmed the presence of an unusual protein. By isoelectric focusing we identified it as egg-white, a finding confirmed by immunofixation wi...

One hundred and sixty–four feacal samples from six different kinds of animals (chickens, cow, pigs, goats, cats dogs) were examined for enterotoxin producing Escherichia coli Enetrohaemorhagic strain 0157:H7, using Suckling mouse assay, ligated rabbit illeal loop assay 0157:H7 antiserum respectively. A total 90(54.9%) strains isolated. EHEC was not detected, but 6(6.7%) isolates found positive ...