Trigalactosyl diglyceride was isolated from leaves of Urtica dioica and characterized by thin layer chromatography, infrared spectroscopy and by its fatty acid composition. An antiserum to the trigalactolipid was obtained by immunization of rabbits. By means of inhibition experiments with oligosaccharides and mono- and digalactosyl glycerol it was demonstrated that the antibodies are directed t...

Two rabbits were immunized with native DNA modified in vitro by cis-dichlorodiammineplatinum(II) (cis-Pt). The interactions between the antiserum and several natural and synthetic nucleic acids were studied primarily by radioimmunoassays. Native DNA's modified by platinum compounds with labile groups in the cis position are recognized by the antiserum. No cross-reaction is found with native DNA...

An adult chimpanzee (Pan troglodyte) with an endogenous circulating carcinoembryonic antigen (CEA) level of 60 ng/ml was immunized s.c. with human CEA. After 1 year of immunizations, the anti-human CEA antibody titer had plateaued. This chimpanzee antiserum demonstrated high avidity specific recognition of human CEA and showed ionic strength effects for CEA recognition similar to those previous...

1. Antiserum was raised against purified Wistar-rat liver UDP-glucuronyltransferase. 2. UDP-glucuronyltransferase activities towards 4-nitrophenol, bilirubin, 1-naphthol and morphine were co-immunoprecipitated from solubilized Wistar-rat liver preparations. 3. UDP-glucuronyltransferase activities towards 1-naphthol, 2-aminophenol and 4-nitrophenol were precipitated from solubilized Gunn-rat liv...

Elevated plasma homocysteine (homocysteinemia) are presumed to be responsible for the development of coronary artery disease, however, the precise etiology is unclear. We examined the possibility that the adduct formed from the reaction between homocysteine thiolactone, a metabolic product of homocysteine, and apolipoprotein B-100 lysyl residues of low density lipoprotein (LDL) was immunogenic....

The management of scorpion stings, especially those including antivenom treatment, remains a subject of controversy. The purpose of this study was to offer the epidemiologic and clinical characteristics of patients with scorpion sting and to determine the efficacy of antivenom treatment in the management of these patients. A total of 106 adult patients admitted for scorpion sting to our ED betw...

Antibodies to neoxanthin agglutinate stroma-free swellable chloroplasts from tobacco (Nicotiana tabacum var. John William's Broadleaf) and Antirrhinum (Antirrhinum majus) whereas stroma-freed chloroplasts, which have lost the swellability are not agglutinated despite the fact that antibodies to neoxanthin are specifically absorbed. In this latter case the agglutination is hindered for sterical ...

The teratogenicity of rabbit anti-rat visceral yolk-sac antiserum, injected into pregnant rats at either 8-5 or 9-5 days of gestation, has been confirmed. Normal rabbit serum was found not to be teratogenic. When conceptuses from 9-5-day pregnant rats were cultured for 48 h in heat-denatured homologous serum, to which antiserum was added for the final (or the penultimate) 6 h of culture, embryo...

Previous investigations from our laboratory show that up-regulation of neuronal nitric oxide synthase (NOS) following spinal cord injury (SCI) is injurious to the cord. Antiserum to dynorphin A (1-17) induces marked neuroprotection in our model of SCI, indicating an interaction between dynorphin and NOS regulation. The present investigation was undertaken to find out whether topical application...

Rhesus monkeys were immunized with normal human lymphoid cells, cultured lymphoid cells, and chronic leukemic lymphocytes. Antisera were analyzed by cytotoxicity and immunofluorescence techniques to study the antigenic characteristics of human lymphocytes. In an attempt to obtain a reagent specifically reactive with T (thymus-derived) lymphocytes, an antispleen antiserum was absorbed with cellf...