For clinical purposes, pure protein and identification of carbohydrate structure from recombinant erythropoietin are needed. Purification was done by Immobilized Metal Affinity Chromatography (IMAC) column charged with Ni (His-Trap affinity chromatography) and continued with gel filtration chromatography column to get purer protein. The carbohydrate group which is oligosaccharide from the resul...

We describe a fully automated method of analysis for plasma epinephrine and norepinephrine by use of a two-column system of "high-performance" liquid chromatography. Catecholamines in deproteinized plasma are purified on the first (preparation) column, then transferred automatically to the second (analytical) column in which epinephrine and norepinephrine are resolved. These compounds are then ...

A selective and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the determination of berberine, palmatine and jatrorrhizine in rat plasma. Target compounds, together with the internal standard (metronidazole), were extracted from rat plasma samples by protein precipitation with acetonitrile-methanol (1:2, v/v). Chromatography was carried out using a ...

Separation of the enantiomers of new chiral alkynes in strategic syntheses and bioorthogonal studies is always problematic. The chiral column high-performance liquid chromatography (HPLC) method in general could not be directly used to resolve such substrates, since the differentiation of the alkyne segment with the other alkane/alkene segment is not significant in the stationary phase, and the...

An uncontrolled used of antibiotics and sulphonamides is responsible for the presence of residues in beehive products and contributes to the problem of food safety. The optimisation of analytical methods for the detection of residues of antibacterial drugs (sulphonamides, SPs; tylosin, TL; streptomycin, ST) in honey was pursued and the methods applied to routine samples. ST was investigated by ...

The protein-keratan sulfate core of bovine nasal cartilage proteoglycan was purified by affinity chromatography on a column of immobilized hyaluronic acid. The hyaluronic acid was immobilized by reaction with a hydrazido-alkyl derivative of Sepharose in the presence of borohydride. Proteoglycan was digested with chondroitinase ABC and the entire mixture was passed over a column of the Sepharose...

A selective and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the determination of berberine, palmatine and jatrorrhizine in rat plasma. Target compounds, together with the internal standard (metronidazole), were extracted from rat plasma samples by protein precipitation with acetonitrile-methanol (1:2, v/v). Chromatography was carried out using a ...

       From hexanic extract of aerial parts of Peucedanum ruthenicum M. Bieb (Apiaceae) collected from Mazandaran province of Iran, four triterpenoids namely taraxasterol, y-taraxasterol, poriferasterol and b-sitosterol have been isolated by column chromatography, thin layer chromatography, and crystallization. Their structures were elucidated by spectroscopic methods (UV, IR, MS, 1H and 13C-N...

In this work, new acrylate cyclotriphosphazene moiety (PN-A) was successfully synthesized starting from (PN) compound. This compound reacted with 2-hydroxyethylacrylate utilizing 1:1 mole ratio to increase the priority of one substitution reaction and forming PN-A molecule. The crud product purified by recrystallization using n-hexane column chromatography cyclohexane: ethyl acetate 60:40 mL as...