A novel protocol for production of multiple shoot tip clumps of Keelar nelli (Phyllanthus amarus Schum. & Thom.) were developed in vitro from shoot tip explants cultured on Murashige and Skoog (MS) medium containing only BAP or BAP with NAA, 3% sucrose and 0.8% agar. The addition of the following combination of growth substances 0.1mgLBAP and 0.05mgLNAA, caused an extensive proliferation of mul...

Embryo culture were used to produce inter specific walnut hybrid plants between Juglans nigra L. and J. regia L. Developed ovaries were collected from J. rejia female flowers which were pollinated with J. nigra pollen grain by high dusting on a mature tree. In order to isolate embryos, exocarps were aseptically removed. Isolated embryos from ovules at different developmental stages were transfe...

An efficient protocol was developed for high frequency plant regeneration from leaf explants of Ammi majus. Ammi majus L. (Apiaceae) is a herb of pharmaceutical as well as ornamental interest and used in the treatment of leucoderma. Since in Ammi majus the seed set and germination is poor attempts were made to propagate the plants through tissue culture. The callus from cotyledonary leaves obta...

So far there is no information about receiving red beet androgenic embryos by androgenesis. Several factors were tested which affected this process: starch accumulation in microspores, correlation between bud length and microsporogenesis course, induction and regeneration medium composition. Ploidy level of obtained regenerants were evaluated. Treating anthers with α-amylase or watering donor p...

Multiple shoots were induced from leaf explants of Lycopersicon esculentum cultivar MicroTom, within 20-25d, on MS medium supplemented with 8.9 microM benzylaminopurine (BAP)+1.14 microM indole-3-acetic acid (IAA). For rooting, elongated microshoots were excised and transferred onto MS medium supplemented with 4.9 microM indole-3-butyric acid (IBA). Well-developed roots and flower raceme were o...

To develop an alternative method for production of sarsasapogenin by plant tissue culture technique and its quantification in Asparagus racemosus and its in vitro cultures using HPTLC. Murashige and Skoog’s (MS) basal medium supplemented with various growth regulators was used for development of shoot and root calli while, hormone free MS medium was used for development of Agrobacterium tumefac...

considering great medicinal value of hyssopus officinalis l. and possibility of its mass production through in vitro culture, two individual experiments was conducted. effect of various concentrations (0, 2.2, 4.4 and 11 µmol) of tdz and bap in combination with 1 µmol of iaa on direct regeneration from nodal explants were assessed. significant difference between treatments was observed (p≤ 0.01...

Functional magnetic resonance imaging (fMRI) was used to examine the processing of infrequent changes occurring in an unattended sound sequence. In event-related brain potentials (ERPs), such sound changes typically elicit several responses, including an enhanced N1, the mismatch negativity (MMN), and the P3a. In the present study, subjects were presented with a repeating sound of 75 ms in dura...