The Neurospora crassa assimilatory nitrite reductase (EC 1.6.6.4) catalyzes the NADPH-dependent reduction of nitrite to ammonia, a 6-electron transfer reaction. Highly purified preparations of this enzyme exhibit absorption spectra which suggest the presence of a heme component (wavelength maxima for oxidized senzyme: 390 and 578 nm). There is a close correspondence between nitrite reductase ac...

A simple, rapid, and environmentally-friendly spectrophotometric method for nitrite detection was developed. Detection based on a redox reaction with iodide ions in an acidic condition. The evaluated by detecting the increase absorbance of colored product iodine at 362 nm wavelength. To obtain good performance, concentration, hydrochloric acid time were optimized. In optimal condition, develope...

A Desulfovibrio vulgaris Hildenborough mutant lacking the nrfA gene for the catalytic subunit of periplasmic cytochrome c nitrite reductase (NrfHA) was constructed. In mid-log phase, growth of the wild type in medium containing lactate and sulfate was inhibited by 10 mM nitrite, whereas 0.6 mM nitrite inhibited the nrfA mutant. Lower concentrations (0.04 mM) inhibited the growth of both mutant ...

It is well known that nitrate is an important nutrient that supports plant growth and development. The application of nitrate also causes extensive changes in the expression of genes coding for proteins involved in nitrogen (N) metabolism. Indeed, genomic analyses have provided a comprehensive dataset of more than a thousand nitrate-responsive genes in Arabidopsis (Arabidopsis thaliana). Much l...

In this paper, the degradation of three endocrine-disrupting chemicals (EDCs): bisphenol A (BPA), 17β-estradiol (E2) and 17α-ethinylestradiol (EE2) by manganite (γ-MnOOH) activated peroxymonosulfate (PMS) was investigated. Preliminary optimisation experiments showed that complete EDCs achieved after 30 min reaction using 0.1 g L−1 γ-MnOOH 2 mM PMS. The rate constants were determined to be 0.20,...