Simultaneous determination of difloxacin and enrofloxacin using two chemometric methods, partial least squares regression (PLS) and direct orthogonal signal correction-partial least squares regression (DOSC-PLS) is described in this paper. The simultaneous determination of these drugs is difficult due to spectral interferences. The calibration graphs were linear in the range of 1-10 μg mL-1 and...

In this research, a rapid, simple and accurate spectrophotometric approach was described for the estimation of phenylephrine hydrochloride in pure its drug forms. The suggested method based on oxidative coupling reaction with p-aminobenzophenone using potassium periodate as an oxidant. A taupe-red dye formed at room temperature showed maximum absorption 512 nm. linearity standard calibration cu...

A Simple, accurate and reproducible spectrophotometric method by Hydrotrophic Solubilisation was developed for the estimation of Esomeprazole. In the present investigation, hydrotropic solution of urea (10M) was employed as a solubilising agent to solubilise the poorly water soluble drug, esomeprazole in the tablet form and determined with the help of spectrophotometric determination in ultravi...

Cartilage glycosaminoglycans (GAGs) were measured by a spectrophotometric assay in synovial fluid obtained from 30 normal bovine hock joints and 15 osteoarthritic human knee joints. Results were compared with those obtained by radioimmunoassay (RIA). The spectrophotometric method (dimethylmethylene blue (DMB) assay) was found to be simple, safe, and sufficiently reproducible to be of potential ...

A simple and derivative spectrophotometric method has been developed for the determination of mercury (II) using Diacetylmonoxime benzoylhydrazone (DMBH). Mercury (II) forms an orange coloured water soluble complex with the reagent in basic medium (pH 10.5).The molar absorptivity and Sandell’s sensitivity of coloured species are 2.01 x 10 L.mol cm and 0.0099 μg/cm respectively. Beer’s law is ob...

A rapid and sensitive direct spectrophotometric method for the determination of cerebrospinal fluid protein which usesas little as 0.1 ml. of sample has been described. The result is not affected by the ratio of albumin to globulin in the sample. Interfering substances are removed prior to spectrophotometric measurement with an automatic separation chamber employing the principle of gel filtrat...