Occurrence of the αS1-casein D allele (formerly "welsh" variant) in sheep breeds Tsigai, Improved Valachian and Slovak Merino was investigated by means of isoelectric focusing in ultra thin polyacrylamide gel. This variant in herds of the above mentioned breeds was observed as follows: Improved Valachian 3.64%; Tsigai 5.57%; Slovak Merino 12.45%. In the last mentioned breed in a single animal α...

named yimelysin, was purified from the cutture supernatant by three column chromatographies. About 150mg of purified vime]ysin -'as obtained from 3.3 Iiters of the culture supernatant with a high yield of 57'ZF. The purified vimelysin showed a single protein band on SDS-PAGE with molecular vveight of 38,OOO. The isoelectric point of vimel)'sin was 4.3 by isoelectric focusing. The optimum pH of ...

Casein is almost insoluble at around pH 4.6, which is its isoelectric point (pI). Grafting copolymer, casein-g-dextran, was prepared through the Amadori rearrangement of the Maillard reaction. The copolymer has a reversible pH sensitive property: micellization at the pI of casein forming a casein core and dextran shell structure and dissociation when pH differs from the pI. The micelles produce...

The study of casein, the principal protein in milk, is being given increasing attention in research laboratories, not only because of the promising new ways in which it may be used in industry, but also because it affords a suitable material for the study of fundamental problems in protein chemistry. Strong acid and alkaline reagents have generally been used as solvents for casein, but they are...

The self-assembly of β-casein and lysozyme, a linear and a globular protein with isoelectric point of pH 5.0 and 10.7, respectively, was studied. Polydisperse electrostatic complex micelles formed when mixing β-casein and lysozyme aqueous solutions. After the micelle solution was heated, lysozyme gelated and β-casein was trapped in the gel, producing narrowly dispersed nanoparticles. The nanopa...

A novel acid proteinase (Tropiase) was isolated from Candida tropicalis IFO 0589 by DE52-cellulose, and DEAE-Cosmogel column chromatographies. The purified tropiase gave a single band on disc polyacrylamide gel electrophoresis, isoelectric focusing and sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. The enzyme preparation had a molecular weight of 23,900, isoelectric point of p...

the κ-casein protein plays a fundamental role in milk production and its composition synthesis in the mammary gland. this protein has an important role in the formation, stabilization, and aggregation of the casein micelles. in this study, we investigated the genetic variability at the exon 4 of the κ-casein gene (also known as csn3) using pcr-sscp analysis and dna sequencing. then a protein se...

Acidification of milk destabilizes casein micelles (CMs) and results in network formation. This process is fundamental during the manufacturing dairy gels such as cheese yoghurts. Understanding structural alterations CMs this aids to predict physicochemical sensory properties gels. Herein we utilize direct Stochastic Optical Reconstruction Microscopy (dSTORM) visualize individual hydrated chara...

1. Milk proteins of the Galapagos fur seal (Arctocephalus galapagoensis) were separated adequately into whey and casein fractions using bovine milk analysis methods. 2. In samples from days 5-30 of lactation 40% of the total proteins were whey and 60% caseins; in mid-lactation, day 150, 25% were whey and 75% casein proteins. 3. Electrophoretic and isoelectric focusing patterns of fur seal whey ...