Simultaneous spectrophotometric determination of salicylamide and paracetamol by H-point standard addition method (HPSAM) and partial least squares (PLS) calibration is described. The results showed that simultaneous determinations could be performed with the ratio 0.2:5–20:1 for salicylamide – paracetamol. A partial least – squares multivariate calibration method for the analysis of binary mix...

a simple, sensitive and economically viable spectrophotometric method for the determination of corticosteroids (predinsolone {psl}, dexamethasone {dex}, prednisone {ps}, betamethasone sodium phosphate {bep} and hydrocortisone {hyd} has been developed. the method involves the oxidation of corticosteroids by iron (iii) and subsequent complexation of iron (ii) with potassium hexacynoferrate (iii),...

the aim of this work was to develop a new and simple coacervative extraction method for the preconcentration and spectrophotometric determination of cu(ii) in water samples. dithizone was used as the chelating agent while an anionic surfactant, namely sodium dodecyl sulfate (sds), was used as extracting agent at room temperature. central composite design (ccd) based on response surface methodol...

A mixture of acetaminophen, diphenhydramine hydrochloride and pseudoephedrine hydrochloride is used for the symptomatic treatment of common cold. In this study, a derivative spectrophotometric method based on zero-crossing technique was proposed for simultaneous determination of acetaminophen, diphenhydramine hydrochloride and pseudoephedrine hydrochloride. Determination of these drugs was perf...

a mixture of acetaminophen, diphenhydramine hydrochloride and pseudoephedrine hydrochloride is used for the symptomatic treatment of common cold. in this study, a derivative spectrophotometric method based on zero-crossing technique was proposed for simultaneous determination of acetaminophen, diphenhydramine hydrochloride and pseudoephedrine hydrochloride. determination of these drugs was perf...

Although the Liley spectrophotometric estimation of bile pigments in amniotic fluid [Amer. J. Obstet. Gynecol. 82, 1359 (1961)] is widely used, the levels of accuracy and precision necessary for clinically meaningful results have not been clarified. This paper delineates some of the important in strumental and procedural sources of error, and how each affects the final value of A451: (a) A limi...

Colored microspheres have become popular compared with radioactive microspheres because they do not use radioactivity. However, they suffer from a much greater variability in their determination. We have developed a new method for assaying the dye using high-performance liquid chromatography (HPLC) with internal standard. This technique permits accurate determination of ≤400 spheres in rat bloo...

Reversed-phase HPLC and UV spectrophotometric techniques using water as solvent have been developed and validated for the simultaneous determination of amoxicillin and cloxacillin in capsules. For both techniques, the linearity range of 60.073x2013;140.0 µg/mL was studied. The spectrophotometric data show that non-derivative techniques, such as absorbance ratio and compensation, and ratio spect...

Several methods are available in literature for the determination of percentage purity of diazepam (pharmaceutical raw material) either by non aqueous titration or spectrophotometric methods. For the quantitative determination of diazepam from the commercial pharmaceutical preparations, the current official B.P method (nonaqueous titration and spectrophotometric assay) has been utilized and a c...

In this study a sensitive, simple and accurate spectrophotometric method was suggested for determination of tamsulosin in bulk powder and pharmaceutical dosage form based on the formation of an ion-pair complex between the drug and bromocresol green in a buffer solution at pH 3.5. The formed yellow color complex was extracted with chloroform and measured at 415 nm. The optimum reaction conditio...