Aconitum is a medicinal treasure trove that grows extensively on fertile pastures in Xinjiang Province (China); however, its molecular genetic characteristics are still poorly studied. We studied Aconitum kusnezoffii Reichb., Aconitum soongaricum Stapf., Aconitum carmichaelii Debx. and Aconitum leucostomum Worosch, using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (...

DNA based molecular markers allow precise, objective and rapid cultivar identification. In this study, five ISSR and two sets of fluorescence-labeled TP-M13-SSR markers were applied for discrimination of eight closely related wheat cultivars. Five ISSR primers revealed a total of 43 distinct reproducible bands, 29 (67.44%) of the 43 bands were polymorphic. The number of polymorphic bands detect...

Pecan is an important horticultural nut crop originally from North America and now widely cultivated in China for its high ecological, ornamental and economic value. Currently, there are over one hundred cultivars grown in China, including introduced American cultivars and Chinese seedling breeding cultivars. Molecular markers were used to assess the genetic diversity of these cultivars and to ...

The genus Salicornia (Amaranthaceae) was established by Linnaeus. Commonly known as ‘glassworts’, the species of are articulated succulent herbs with cortical palisade, opposite decussate scale-leaves, thyrsoid cymes, flowers packed in cauline depressions and diaspore composed l-seeded utricle. Therefore, due to importance plant species, we performed a combination morphological molecular data a...

Analysis of the extent and distribution of genetic diversity in crop plants is essential for optimizing sampling and breeding strategies. Several methods were identified for the existence of diversity in maize for the aim of perfect selection based on great diversity among maize genotypes. In this study, Inter-simple sequence repeat (ISSR) markers were used to assess genetic diversity in a sele...

The genetic relationship among some naturally growing Vicia taxa in Turkey (V. sativa subsp. sativa, subsp.nigra var.nigra, subspincisavar.cordata, V. craccasubsp.cracca, subspgerardii, subspatroviolacea, subspstenophylla, V. hybrida, V. peregrinaandV. palaestina) was assessed by helping ISSR markers. During PCR assays, although 17 primers were tested, only three primers produced informatics am...

Dog Rose is one of the most important species in Rosaceae family used as a medicinal plant and a rootstock for ornamental roses. This species is native to Iran, therefore, identification of indigenous genotypes of this species is important for genetic preservation or breeding purposes. Genetic diversity estimation of plant materials is one of the important pre-breeding activities in breeding cr...

Necrophagous fly is the most common insect evidence collected during a death investigation. We analyzed the DNA polymorphism among the five forensically important fly species, namely, Lucilia sericata, Aldrichina grahami, Chrysomya megacephala, Parasarcophaga crassipalpis and Musca domestica using inter simple sequence repeat (ISSR) method. Nine ISSR primers selected from 18 primers could ampli...

The variability level of the ISSR (inter-simple sequences repeat) primer (GACA)4 was examined in the three Lepidoptera families Pyralidae, Sphingidae and Pieridae. Our study shows that the tetra-repeat (GACA)n is evidently present in sufficient numbers in these butterflies to provide informative DNA fingerprints. The variability is mostly rather high, but within a comparable range to other ISSR...

Three different DNA-based techniques, Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeat (ISSR) and Amplified Fragment Length Polymorphism (AFLP) markers, were used for fingerprinting Dactylis glomerata genotypes and for detecting genetic variation between the three different subspecies. In this study, RAPD assays produced 97 bands, of which 40 were polymorphic (41.2%). The I...