Melia volkensii Gurke is a drought-tolerant tree native to East Africa's arid and semiarid lands (ASALs), with vast but underutilized potential for agroforestry and sustainable livelihoods in the ASALs. Its cultivation is limited by difficulties in propagation via conventional means. Full exploitation of the ability of thidiazuron (TDZ) to elicit regeneration in plant tissue cultures, as sole p...

Andrographis lineata Nees (Acanthaceae) is an endemic herb rich in flavonoids and diterpenoids which have pharmaceutical importance. Seedling explants source has not been studied for micropropagation and hence the present investigation has been taken up. An efficient protocol was developed for aseptic seed germination and in vitro shoot multiplication from different aseptic seedling explants. 3...

Genetic variation due to somaclonal variation in micropropagated plants is a beneficial phenomenon for crop improvement. Genetic integrity of the plants derived through micropropagation becomes crucial if genetic transformation studies have to be carried out. Somaclonal variation in tissue culture is a common phenomenon which makes it mandatory to check for genetic stability of plants. Hypocoty...

In vitro seedlings were used as explants for protocorm like bodies (PLBs) production which in turn were used for regeneration purpose. PLBs were induced from the base of seedlings (1.0-1.5 cm in size) in MS + BAP (8.88 microM). After 90 days of inoculation, PLBs production rate started declining and most of the PLBs turned into plantlets. Preculture of seedlings in 1.0 microM thidiazuron (TDZ) ...

This study was conducted to determine the effect of compost and compost tea on growth and physiological parameters of Lepidium sativum L. 30-day-old Cress plants treated with compost and compost tea were harvested and different growth and physiological parameters were measured. Compost tea application increased RGR, SLA, ULR and RLAGR. The contents of Chlorophyll a and Chlorophyll b in the plan...

background and objectives: bitter melon is one of the anti-cancer medicinal plants that it’s wide level in vitro propagation and anti-cancer material production was necessary. different factors such as genotype, selected explants’ type and it`s preparation conditions influenced regeneration content. plant growth regulator`s effect on callogenesis and indirect regeneration from parangipetai indi...

Dendrobium phalaenopsis Fitzg. (also known as the Larat orchid) is an endemic orchid from Island, Eastern Indonesia. Its beautiful flowers mean that many plants are taken for commercial purposes, leading to rapid decline of populations in their natural habitats. The objectives this study were determine which organs transgenic carrying 35S::GR::AtRKD4 construct, together with concentrations plan...

Somatic embryogenesis was induced in hypocotyl explants of geranium (Pelargonium x hortorum) cultured on media supplemented with various concentrations of N-phenyl-N'-1,2,3-thiadiazol-5-ylurea (thidiazuron). In less than 2 weeks, somatic embryos were observed in treatments containing levels of thidiazuron (TDZ) ranging from 0.2 to 1.0 micromolar. The use of N(6)-benzylaminopurine in combination...

in order to optimize callus induction in pistacia atlantica desf. subsp. kurdica rech. f., the effect of different plant growth regulator (pgr) combinations were studied. inflorescence axis explants were cultured on wpm medium supplemented with 1, 2 and 3 mg/l of tdz or 1, 2 and 3 mg/l of ba, in combination with 1mg/l different auxins: 2, 4-d,  naa  and iba . callus initiation was observed in a...