The formation of a biofilm by many opportunistic bacteria such as Pseudomonas aeruginosa has been shown to provide the bacteria with a form of protection from various environmental fluctuations. Biofilm formation through the excretion of an exopolysaccharide matrix has become particularly important in hospital settings where many Pseudomonas infections are resistant to commonly used antibiotics...

The effect of graphene oxide (GO) or reduced GO (rGO) on the structure and properties polyelectrolyte-complexed chitosan/alginate bionanocomposites is highly dependent plasticiser type (glycerol 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc])) due to competing interactions between components. For glycerol-plasticised matrix, inclusion GO/rGO enhanced chitosan crystallinity increased matrix d...

background: the problem of bone defects during treatments of bone lesions, especially cranial trauma, and also during craniotomy operations indicates isolation of osteoblasts and culturing them for filling these bone effects. methods: bone tissue specimens were obtained from 6 patients’ carvaria undergoing craniotomy surgery operations in al-zahra teaching hospital and transferred to lab. these...

BACKGROUND The three-dimensional (3D) system is one of the important factors to engineer a biocompatible and functional scaffold for the applications of cell-based therapies for cartilage repair. The 3D alginate hydrogels system has previously been shown to potentially promote chondrogenesis. The chondrocytic differentiation of co-cultured adipose-derived stem cells (ADSCs) and nasal chondrocyt...

Title of Thesis: Recirculation Flow Bioreactor for the Synthesis of Nitroxide and N-Oxide Pharmaceuticals Douglas Lischick: Master of Science in Chemical Engineering, 1989 Thesis directed by: Sam S. Sofer, Professor Sponsored Chair in Biotechnology An investigation of the properties of calcium alginate immobilized hog liver microsomes, rich in FAD-containing mixed function oxidase, was performe...

The extracellular biofilm matrix includes primarily DNA and exopolysaccharides (EPS), which function to maintain aggregate structures and to protect biofilms from antibiotics and the immune response. Both polymers are anionic and have cation binding activity, however the impact of this activity on biofilms is not fully understood. Host cell contact is considered the primary signal for activatio...

Two probiotic bacteria (Lb. acidophilus and B. lactis) were immobilized on the alginate coat of strawberry pieces and were added to the prepared sweet non fat dietary stirred yogurt. Physico-chemical parameters namely pH value, titratable acidity and synersis along with the viability of probiotic bacteria of the samples were evaluated over the 8 days of storage at 5 ºC at 2 day intervals. A sig...

One of the major challenges in regenerative medicine is the ability to recreate the stem cell niche, which is defined by its signaling molecules, the creation of cytokine gradients, and the modulation of matrix stiffness. A wide range of scaffolds has been developed in order to recapitulate the stem cell niche, among them hydrogels. This paper reports the development of a new silk-alginate base...

INTRODUCTION Alginate hydrogel is an advantageous scaffold material for cartilage tissue engineering because it encapsulates cells homogenously and provides intrinsic porosity. Blending alginate with cell adhesive molecules such as type I collagen to make composite hydrogels can further promote chondrogenenesis of mesenchymal stem cells. However, in these conventional composites, the collagen p...

Encapsulation of probiotic bacteria with a matrix can increase their survival rate by protecting them from adverse conditions and at the same time without affecting the production of metabolites. An effort has been made to encapsulate the probiotic Lactobacillus plantarum using calcium alginate. Box-Behnken model of response surface methodology (RSM) was employed in the optimization of major en...