BACKGROUND Adenovirus vectors have been utilized for cancer gene therapies. The present study examined the oncolytic effects of adenovirus type 5 (Ad5) and fiber-substituted conditionally replicating adenovirus (CRAD) Ad5/F35 vectors on the human malignant mesothelioma cells MSTO-211H, NCI-H28, NCI-H2052, and NCI-H2452 cells. MATERIALS AND METHOD For the adenovirus, the first mRNA/protein to ...

2014 We investigate the possibility to observe Doppler-free two-photon transitions in the case where one of the transitions is an electric dipole transition and the other one is a magnetic dipole or an electric quadrupole transition. We show that it is not possible to obtain Doppler-free lines by reflecting the beam on itself. On the other hand, such an effect should be observable if the two co...

Using quantitative gel retardation assays the properties of the bovine papilloma virus (BPV) origin recognition protein E1 and the effect of the viral E2 protein on the binding of E1 to BPV origin DNA were examined. As reported previously (Seo, Y.S., Mueller, F., Lusky, M., Gibbs, E., Kim, H.-Y., Phillips, B. and J. Hurwitz (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 2865-2869), the E1 protein b...

Microsomal epoxide hydrolase (mEH, EPHX1) is a critical xenobiotic-metabolizing enzyme, catalyzing both detoxification and bioactivation reactions that direct the disposition of chemical epoxides, including the carcinogenic metabolites of several polycyclic aromatic hydrocarbons. Recently, we discovered that a previously unrecognized and primate-specific EPHX1 transcript, termed E1-b, was actua...

The full-length product of the bovine papillomavirus type 1 (BPV-1) E1 translational open reading frame is required for viral DNA replication in vivo and in vitro. E1 is a multifunctional protein whose properties include ATP binding, acting as an ATPase-dependent DNA helicase, DNA binding to the BPV-1 origin of viral DNA replication, and association with the E2 transcriptional transactivator, E...

Let G(V,E1) and G (V,E2) be two networks on the same vertex set V and consider the union of edges G(V,E1 ∪ E2). This paper studies the stability of the Degree, Betweenness and Eigenvector Centrality of the resultant network, G(V,E1 ∪ E2). Specifically assume v max and v max are the highest centrality vertices of G(V,E1) and G(V,E1 ∪ E2) respectively, we want to find Pr(v max = v max). ar X iv :...

Plant expression of thermostable endoglucanase (E1) [pdb:1ECE] has been proposed for improved conversion of lignocellulose to ethanol for fuel production. Since E1 was isolated from hot springs and has thermostable properties, it may have increased stability when released to agricultural soils during post-harvest residue decomposition. Endoglucanases are a subclass of cellulases and act by clea...

The mechanism of DNA replication is conserved among papillomaviruses. The virus-encoded E1 and E2 proteins collaborate to target the origin and recruit host DNA replication proteins. Expression vectors of E1 and E2 proteins support homologous and heterologous papillomaviral origin replication in transiently transfected cells. Viral proteins from different genotypes can also collaborate, albeit ...

AIMS Folate receptors (FRs) mediate cellular uptake of folates in many cancer cells and in folate deficiency heterogeneous nuclear ribonucleoprotein E1 (hnRNP-E1) mediates translational upregulation of FR in cultured cervical cancer cells. hnRNP-E1 can also interfere with human papillomavirus 16 (HPV-16) viral capsid protein synthesis (and thereby HPV proliferation) in vitro. This study aimed t...

UNLABELLED The measure of changes in synaptic dopamine (DA) concentration in response to the psychostimulant drug methylphenidate (MP) has been used as an indicator of responsiveness of the DA system. The purpose of this study was to assess the reproducibility of these measures. METHODS Seven healthy subjects were scanned with PET and [11C]raclopride twice in the same day: 7 min after placebo...