Following preparative isolation and purification by high-speed counter-current chromatography (HSCCC), the collected fractions were generally analyzed by high-performance liquid chromatography (HPLC) to determine the relative purities of each fraction. Our paper reports for the first time a preparative isolation-purity detection hyphenated system: online coupling of HSCCC with high-performance ...

The roles of isoflavones in the prevention of several hormone-dependent cancers and osteoporosis are of great interest. Despite many pharmacokinetics studies of the isoflavones, the actual types of conjugates circulating in the body and the position(s) of conjugation sites on the flavone skeleton are still uncertain because, in general, conjugated compounds in biological fluids have been evalua...

BACKGROUND Boswellia serrata, also known as Indian frankincense is a commercially important medicinal plant which has been used for hundreds of years as an Ayurvedic medicine for the attempted treatment of arthritis. It contains naturally occurring triterpenoic acids, called as boswellic acids (BA's). RESULTS A highly reproducible High performance liquid chromatography-ultraviolet diode array...

• Effective extraction of fucoxanthin, ?-carotene and chlorophyll-a from brown algae. Development a simple HPLC method for detecting algae pigments. Rapid identification quantification pigments by HPLC-DAD. Assessment the best techniques to obtain highest yield. Efficient pigment with UAE nine species. Seaweeds are photosynthetic organisms that have high contents The coloration each alga is def...

Objective. Oxidative stress leads to numerous physiological disorders including infectious diseases, inflammation, and cancer. The present study was carried out to investigate antioxidant, antibacterial, and cytotoxic activity of methanol crude extract of leaves and fruits of the Ficus racemosa (LCME and FCME, resp.) and to analyse its major bioactive polyphenols by HPLC-DAD. Methods. Antioxida...

Pistachio hull contains anacardic acids. It is a salicylic acid with side chain either saturated or unsaturated. This work focused on the efficient extraction of acids using solvent. Six aliquots were weighed and extracted hexane, ethyl acetate, mixture H2O/MeOH/HCl. Hexane was most solvent due to its ability dissolve Only one standard used quantify all them since does not affect wavelength gre...

The aerial parts of several Haplopappus species (Asteraceae), known under the common name "baylahuen", are used as herbal teas in Chile and Argentina. In Chile, "baylahuen" comprises H. multifolius, H. taeda, H. baylahuen and H. rigidus. Little is known about the chemical identity of the infusion constituents in spite of widespread consumption. The aim of the present work was the characterizati...