D ow nladed f Carvedilol selectively inhibits oscillatory intracellular calcium changes evoked by human a1Dand a1B-adrenergic receptors Taka-aki Koshimizu*, Gozoh Tsujimoto, Akira Hirasawa, Yoko Kitagawa, Akito Tanoue Department of Molecular Pharmacology, National Research Institute for Child Health and Development, 3-35-31, Taishido, Setagaya, Tokyo 154-8567, Japan Department of Genomic Drug D...
Probabilistic projection of climate change consists of formulating the climate change information in a probabilistic manner at either global or regional scale. This can produce useful results for studies of the impact of climate change impact and change mitigation. In the present study, a simple yet effective approach is proposed with the purpose of producing probabilistic results of climate ch...
The structural basis of Ca channel inhibition by G proteins has received considerable attention recently, and multiple regions on Ca channels that interact with G protein subunits have been identified. We have demonstrated previously that a region extending from the N terminus to the I/II loop of the Ca channel is involved in determining the differences between a1B and a1E Ca channels with resp...
We have characterized the pharmacological antagonism, i.e., neutral antagonism or inverse agonism, displayed by a number of a-blockers at two a1-adrenergic receptor (AR) subtypes, a1aand a1b-AR. Constitutively activating mutations were introduced into the a1a-AR at the position homologous to A293 of the a1b-AR where activating mutations were previously described. Twenty-four a-blockers differin...
Walter G. Thomas,
Richard J. Lewis,
Ligand binding and conformational changes that accompany signaling from G protein–coupled receptors (GPCRs) have mostly focused on the role of transmembrane helices and intracellular loop regions. However, recent studies, including several GPCRs cocrystallized with bound ligands, suggest that the extracellular surface (ECS) of GPCRs plays an important role in ligand recognition, selectivity, an...
Damian C. Bell,
Gary J. Stephens,
Karen M. Page,
Annette C. Dolphin,
Voltage-dependent calcium channels (VDCCs) are heteromultimers composed of a pore-forming a1 subunit and auxiliary subunits, including the intracellular b subunit, which has a strong influence on the channel properties. Voltage-dependent inhibitory modulation of neuronal VDCCs occurs primarily by activation of G-proteins and elevation of the free Gbg dimer concentration. Here we have examined t...
Li B. Wang,
This study estimates MJO change under the A1B greenhouse gas emission scenario using the ECHAM5 AGCM whose coupled version (ECHAM5/MPI-OM) has simulated best MJO variance among fourteen CGCMs. The model has a horizontal resolution at T319 (about 40 km) and is forced by the monthly evolving SST derived from the ECHAM5/MPI-OM at a lower resolution of T63 (about 200 km). Two runs are carried out c...
PEDRO J. GONZALEZ-CABRERA,
PATRICK L. IVERSEN,
MARVIN F. LIU,
MARGARET A. SCOFIELD,
WILLIAM B. JEFFRIES,
To investigate a1B-adrenoceptor function, we developed a phosphorothioate antisense oligodeoxynucleotide (AO) to inhibit the expression of the a1B-adrenoceptor subtype in DDT1 MF2 cells. We measured the cellular uptake of the AO and its effect on a1B-adrenoceptor mRNA expression, protein density, and coupling to phospholipase C. Cells treated with either a control oligodeoxynucleotide (CO) or m...
جهت آشکارسازی تغییر اقلیم ابتدا عناصر اقلیمی دما، بارش، فشار تراز دریا و نم ویژه خلیج فارس مشخص گردید و به دو بخش سواحل شمالی و سواحل جنوبی تقسیم گردید. برای ایستگاه ها دوره آماری مشخص (2015-1970) انتخاب گردید و سپس از وبگاه شبکه تغییر اقلیم کانادا داده های ایستگاه شامل (بارش، دما ، ارتفاع ژئوپتانسیل و نم ویژه)اخذ گردید و در مرحله بعد همین روند برای دوره آینده (2050-2016) انجام گرفت. با توجه ب...