نتایج جستجو برای ������������ RAPD

تعداد نتایج: 2752  

2002
Junichi Tanaka, Fumiya Taniguchi,

The random amplified polymorphic DNA (RAPD) technique has been used widely for purposes such as the construction of linkage maps, QTL analysis, evaluation of genetic diversity, and parentage tests. However, some minor bands of RAPD display a low reproducibility and low reliability. We describe a simple and efficient method for making minor RAPD bands clearer. The first step of this method is to...

2015
Ambika B Gaikwad,

RAPD is a PCR based technique which involves the use of single arbitrary. Advantages disadvantages, limitations and applications of RAPD.RAPD is a modification of the PCR in which a single, short and arbitrary. Therefore, RAPD technique can be performed in a moderate laboratory for most of.sophisticated equipment has made the RAPD technique valuable although. Tartflmann odak noktas ise de, RAPD...

2003
Hongwen Huang, Desmond R. Layne,

AIWKKY. Thirty-four extant pawpaw [&imina w&& (L.) Dunsl] cultivars and advancedselections representing a large portion of the gene pool of cultivated pawpaws were investigated using71 randomly amplified polymorphic DNA (RAPD) markers to es tabl i sh genet ic ident i t ies and evaluate genet ic re latedness . All 34 cu l t ivated pawpaws were uniquely identified by as few as 14 loci of eight pr...

Journal: :Nucleic acids research 1992
M P Scott, K M Haymes, S M Williams,

DNA variation from randomly amplified polymorphic DNA (RAPD) (1, 2) has recently been helpful in addressing a wide range of evolutionary problems including the determination of paternity and maternity (3). It has been greeted with particular enthusiasm because it is fast, technically easy and requires little material (4). By using single 10 base primers of arbitrary sequences to amplify DNA wit...

2002
Xingnong Wang, Peter Felker, Mark D. Burow, Andrew H. Paterson,

An analysis of morphological, physiological, and molecular data from five cactus fruit varieties from Mexico and Chile, two ornamental Texas accessions, and one vegetable accession from Mexico are presented. Molecular analysis was performed using the RAPD (random amplification of polymorphic DNA) method. Phenotypic and molecular analyses distinguished ornamental, vegetative, and fruit market ac...

2017
Muhammad Irfan, Shuang Yang, Luo Yuxin, Jia-Xing Sun,

This study investigated the genetic diversity of morchella species using randomly amplified polymorphic DNA markers. Morchella species are an important group of edible mushrooms belonging to the family Helveliaceae with medicinal and economical significance. In this study we have developed an efficient method of genomic DNA isolation which was amplified by eight RAPD primers to test the polymor...

2001
Fevzi BARDAKCI,

Due to advances in molecular biology techniques, large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD markers are amplificatio...

Journal: :BioTechniques 1997
A M Comes, J F Humbert, F Laurent,

The random-amplified polymorphic DNA (RAPD) technique (5,7) is one of the most useful methods for species identification and studies on the genetic structure of populations of microand macro-parasites. This method generally provides numerous markers that must be cloned and labeled to be used as probes (1,4). These probes can be used to test the specificity and the polymorphism of the RAPD marke...

2009
Shalini, Saurabh Tiwari, Rajinder K Gupta, Dolly Wattal Dhar,

Random amplification of polymorphic DNA (RAPD) analysis using the polymerase chain reaction proved a useful technique in the biodiversity analysis of microorganisms but may lack reproducibility in the poorly standardized methodology. In the present investigation, the RAPD technique was optimized for characterizing cyanobacterial isolates in order to ensure its reproducibility and discriminatory...

Journal: :Journal of biochemistry and molecular biology 2002
Mohammad Belayat Hossain, Samiul Haque, Haseena Khan,

The genotype characteristic of cultivars was investigated, along with varieties of both of the jute species, Corchorus olitorius and Corchorus capsularis, in the germplasm collection at the Bangladesh Jute Research Institute (BJRI). DNA fingerprinting was generated for 9 different varieties and 12 accessions of jute cultivars by using random amplified polymorphic DNA (RAPD). A total of 29 arbit...

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