The primary culture of neuronal cells plays an important role in neuroscience. There has long been a need for methods enabling the long-term culture of primary neurons at low density, in defined serum-free medium. However, the lower the cell density, the more difficult it is to maintain the cells in culture. Therefore, we aimed to develop a method for long-term culture of neurons at low density...

To establish a typical tumor model of ovarian cancer which may be more representative and reliable than traditional monolayer culture and pellet, agarose was used as cell vehicle to engineering tumor. Selection of agarose is based on its successful application in tissue engineering with both amenable mechanical and biological properties. In this study, ovarian cancer cell line SKOV3 was encapsu...

To construct tissue-engineered blood vessels (TEBVs) in vitro, it is necessary to transfer seed cells three-dimensional (3D) scaffolds for culture. However, what happens the behavior of after they are transferred scaffold unclear. Therefore, this study, a transcriptome analysis was used characterize differentially expressed genes (DEGs) vascular smooth muscle (VSMCs) before and 3D polyglycolic ...

Two-dimensional (2D) cell cultures growing on plastic do not recapitulate the three dimensional (3D) architecture and complexity of human tumors. More representative models are required for drug discovery and validation. Here, 2D culture and 3D mono- and stromal co-culture models of increasing complexity have been established and cross-comparisons made using three standard cell carcinoma lines:...

introduction: decidua as the nearest mother's tissue layer to the fetus which is in direct contact with the embryo has an important role in composing the appropriate microenvironment for protection of allogenic fetus from mother's immune response. in this study the effect of supernatant from culture of decidual cells on dendritic cells regarding the induction of t-cell allogenic proliferation r...

an essential requirement for an operating pem fuel cell is providing proper water content in the membrane. to avoid water flooding an appropriate water balance is required. here, an analytic model of a planar membrane humidifier for pem fuel cell is proposed where the effect of dimensional parameters includes membrane thickness, membrane area and channel hydraulic diameter are investigated. a n...

objective: the organotypic co-culture is a well-known technique to examine cellular interactions and their roles in stem cell proliferation and differentiation. this study aims to evaluate the effects of dermal fibroblasts (dfs) on epidermal differentiation of adipose-derived stem cells (ascs) using a three-dimensional (3d) organotypic coculture technique. materials and methods: in this experim...

this study was designed to examine the effects of retinoic acid adding to cumulus and/or fibroblast cellsmonolayer on the development of mouse early embryos. one-cell mouse embryos were obtained from nmrimice after superovulation by an intraperitoneal injection of 5 iu equine chorionic gonadotrophin (ecg)followed 48 hrs later by 5 iu human chorionic gonadotrophin (hcg). mouse embryonic fibrobla...

Since the 1970s, the limitations of two dimensional (2D) cell culture and the relevance of appropriate three dimensional (3D) cell systems have become increasingly evident. Extensive effort has thus been made to move cells from a flat world to a 3D environment. While 3D cell culture technologies are meanwhile widely used in academia, 2D culture technologies are still entrenched in the (pharmace...