نتایج جستجو برای: 2 dimensional cell culture
تعداد نتایج: 4287680 فیلتر نتایج به سال:
بررسی میزان بقاء و رشد سلول های بنیادی CD93 بر روی داربست سه بعدی زیست تخریب پلی کاپرولاکتون- ژلاتین
Background and purpose: Application of three-dimensional scaffolds with the ability to simulate a three-dimensional in vivo environment has opened new perspective on targeted differentiation and therapeutic use of stem cells. In this study we examined the compatibility of CD93 stem cells with biodegradable pcl- gelatin scaffold. Materials and methods: In this experimental study, three-dimens...
Cell culture is an important tool for biological research. Two-dimensional cell culture has been used for some time now, but growing cells in flat layers on plastic surfaces does not accurately model the in vivo state. As compared to the two-dimensional case, the three-dimensional (3D) cell culture allows biological cells to grow or interact with their surroundings in all three dimensions thank...
Cells in vivo exist within the context of a multicellular tissue, where their behavior is governed by homo- and heterotypic cell-cell interactions, the material properties of the extracellular matrix, and the distribution of various soluble and physical factors. Most methods currently used to study and manipulate cellular behavior in vitro, however, sacrifice physiological relevance for experim...
Background and purpose: Microfluidic systems are microstructures that could be used to improve the conventional cell culture protocols used in laboratories. The aim of this research was to design and construct the microfluidic system and evaluating its ability to produce IL-2 by jurkat cells. Material and methods: At first, the sketch of microfluidic canals was designed by Corel draw and wa...
survival, proliferation, and migration of human meningioma stem-like cells in a nanopeptide scaffold
objective(s): in order to grow cells in a three-dimensional (3d) microenvironment, self-assembling peptides, such as puramatrix, have emerged with potential to mimic the extracellular matrix. the aim of the present study was to investigate the influence of the self-assembling peptide on the morphology, survival, proliferation rate, migration potential, and differentiation of human meningioma st...
Mammalian cell culture has served as an invaluable tool in cell biology for several decades. Mono layers of adherent cells grown on flat and rigid two-dimensional (2D) substrates, such as polysty rene or glass, have evolved as the mainstay in conventional cell culture systems. Two-dimensional cell culture studies have played a pivotal role in furthering our understanding of developmental biol...
apoptotic and cytotoxicity effects of all trans retinoic acid (atra) in cultured human uterine myoma
background: uterine leiomyoma is the most common tumor among the female in the reproductive period. the first step to control the tumor is employing medical treatment. currently gnrh agonists are the most common drugs for medical therapy of uterine myoma. climacteric-like symptoms and osteoporosis and recurrence of the size of tumor after gnrh agonists consumption are among the most common sid...
background: collagen-based three-dimensional (3d) in vitro systems have been introduced to study the physiological states of cells. as a biomolecule, collagen is usually extracted from terrestrial animals whilst aquatic animals like squid contain large amounts of collagen. methods: in order to make effective use of marine organisms, we selected persian gulf squid in 2015 to extract the required...
nowadays, cell -based and tissue engineered products have opened new horizons in treatment of incurable nervous system disorders. the number of studies on the role of schwann cells (sc) in treating nervous disorders is higher than other cell types. different protocols have been suggested for isolation and expansion of sc which most of them have used multiple growth factors, mitogens and fetal b...
Fetal bovine serum (FBS) has been reported to affect chondrocyte biosynthesis in monolayer culture. Insulin-Transferrin-Selenium (ITS) was investigated as a partial replacement for FBS during in vitro culture of rabbit articular chondrocytes in three-dimensional alginate scaffold. Chondrocyte-seeded alginate hydrogels were cultured in Dulbecco's modified Eagle's medium plus 10% FBS, 1% ITS plus...
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