The metabolism of 5-aminosalicylate (5AS) by a bacterial strain, Pseudomonas sp. BN9, was studied. Intact cells of Pseudomonas sp. BN9 grown with 5AS oxidized 5AS and 2,5-dihydroxybenzoate (gentisate), whereas cells grown with gentisate oxidized only the growth substrate of all substituted salicylates tested. Cell extracts from Pseudomonas sp. BN9 catalysed the stoichiometric reaction of 1 mol ...

The pulmonary cavity is the focus considering the campaign against tuberculosis. The cavity decides the patients fate and dominates the epidemic state of tuberculosis. The persistence of the destructive lesions perpetuates the source of infection. Of people in whom sputum tubercle bacilli are found, more than 50 per cent die within 10 years of the discovery, in spite of collapse therapy and san...

The realization that p-aminobenzoic acid is essential as a growth factor has stimulated extensive research in the field of antagonists of this essential metabolite. Woods (1940) and Woods and Fildes (1940) reported that yeast contained a substance, identified as p-aminobenzoic acid, that could protect it against the growth inhibiting action of sulfanilamide. Youmans et al. (1947) reported the t...

Mineralization potentials, rates, and kinetics of the three phenoxy acid (PA) herbicides, 2,4-dichlorophenoxyacetic acid (2,4-D), 4-chloro-2-methylphenoxyacetic acid (MCPA), and 2-(4-chloro-2-methylphenoxy)propanoic acid (MCPP), were investigated and compared in 15 soils collected from five continents. The mineralization patterns were fitted by zero/linear or exponential growth forms of the thr...

Dihydroflavanol 4-reductase (DFR) is a key later enzyme involved in two polyphenols' (anthocyanins and proanthocyanidins (PAs)) biosynthesis, however it is not characterized in cotton yet. In present reports, a DFR cDNA homolog (designated as GhDFR1) was cloned from developing fibers of upland cotton. Silencing GhDFR1 in cotton by virus-induced gene silencing led to significant decrease in accu...

It has been shown that the enzyme Beta-Glucuronidase Is present in the fluid which develops in the pleural cavity during certain diseases.’ Whether this enzyme occurs as a result of migration through the pleura from the blood, or whether it Is secreted by the epithelium lining the pleural cavity has not been determined. In our studies at this hospital on pleural effusions, we have assayed Beta-...

OBJECTIVE ACPAs are thought to play a pathogenic role in RA. Because of their polyclonal nature it is difficult to study characteristics of ACPAs such as cross-reactivity or affinity. This study aimed to analyse the ACPA response at clonal level. METHODS Citrullinated fibrinogen-specific B cells were isolated from blood derived from an RA patient by fluorescent automated cell sorting (FACS). ...

The purpose of this study was to establish a standardized protocol for second-line antimicrobial susceptibility testing of Mycobacterium tuberculosis using the Bactec MGIT 960 system in Canadian laboratories. Four Canadian public health laboratories compared the susceptibility testing results of 9 second-line antimicrobials between the Bactec 460 and Bactec MGIT 960 systems. Based on the data g...

We describe a colorimetric method for assay of microsomal aminopeptidase (EC 3.4.11.2) activity in serum. We use a new substrate, L-leucyl-3-carboxy-4-hydroxyanilide, p-xylenol as coupler, and sodium metaperiodate as oxidizing reagent. The colored substance formed by the oxidative condensation between p-xylenol and 5-aminosalicylic acid absorbs maximally at 635 nm, and can be directly measured ...

Treatment of multidrug-resistant tuberculosis (MDR-TB) is challenging because of the high toxicity of second-line drugs and the longer treatment duration required compared with drug-susceptible TB. The efficacy of treatment for MDR-TB is poorer than that for drug-susceptible TB. The selection of drugs in MDR-TB is based on previous treatment history, drug susceptibility results, and TB drug res...