Background: During radiation therapy, stromal cells surrounding the tumor (e.g mesenchymal stem cells) may affect the treatment outcomes. We aimed to investigate the effects of gamma radiation on the mRNA expression of cytokines, DNA damage and population doubling time (PDT) of adipose-derived mesenchymal stem cells (ASCs). Material and methods: ASCs were enzymatically extracted from breast tis...

Differentiation of derived from in vitro-incubated adult human mesenchymal stem cells (MSCs), isolated bone marrow, cartilage and adipose tissue, respectively, by application appropriate incubation conditions, to osteoclasts, was investigated proved. The current results show the importance changes mitochondrial dynamics about differentiation male germ (spermatogonia) mature functional activity ...

Objective: In this study, the bone regeneration ability of polyacrylamide hydrogel and nanohydroxyapatite scaffolds (PAAH/NHA) and stem cells derived from adipose tissue (ADSCs) in the healing of critical sized bone defects in rabbit radius were assessed. Animals and procedures: 12 New Zealand white male rabbits were divided into 3 groups. The rabbits were anesthetized and 15 mm bone def...

We examined cell isolation, viability, and growth in adipose-derived stem cells harvested from whole adipose tissue subject to different cryopreservation lengths (2-1159 days) from patients of varying ages (26-62 years). Subcutaneous abdominal adipose tissue was excised during abdominoplasties and was cryopreserved. The viability and number of adipose-derived stem cells isolated were measured a...

background  isolation of adipose tissue mesenchymal stem cells (mscs) with current enzymatic methods has some limitations. for example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of mscs. also, it is accompanied with the increased risk of culture contaminations because of several handling steps.in this ...

BACKGROUND The pericytes in the blood vessel wall have recently been identified to be important in regulating vascular formation, stabilization, remodeling, and function. We isolated and identified pericyte-like platelet-derived growth factor receptor beta-positive (PDGFRβ+) cells from the stromal vascular fraction (SVF) of adipose tissue from critical limb ischemia (CLI) patients and investiga...

Introduction: Human adipose-derived mesenchymal stem cells (hADSCs) are easily accessible in the body, and under appropriate conditions, they can be directed toward various phenotypes. Therefore, hADSCs have been considered as a potential cell source for tissue engineering applications. hADSCs are able to differentiate into endothelial cells which covers the interior surface of vessels, in vi...

Rabbits have the capacity to regenerate holes in their ears by forming a blastema, a tissue that is made up of a group of undifferentiated cells. The purpose of the present study was to isolate and characterize blastema progenitor cells and compare them with marrow mesenchymal stem cells (MSCs). Five New Zealand white male rabbits were used in the present study. A 2-mm hole was created in the a...