Brucella spp. are stealthy bacteria that enter host cells without major perturbation. The molecular mechanism involved is still poorly understood, although numerous studies have been published on this subject. Recently, it was reported that Brucella abortus utilizes cellular prion protein (PrP(C)) to enter the cells and to reach its replicative niche. The molecular mechanisms involved were not ...

Brucellosis caused by Brucella spp. is a major zoonotic disease. Control of brucellosis in agricultural animals is a prerequisite for the prevention of this disease in human beings. Recently, Brucella melitensis was declared by the Centers for Disease Control and Prevention to be one of three major bioterrorist agents due to the expense required for the treatment of human brucellosis patients. ...

Brucellosis is an important zoonotic disease of nearly worldwide distribution. This pathogen causes abortion in cattle and undulant fever, arthritis, endocarditis and meningitis in human. The immune response against B. abortus involves innate and adaptive immunity involving antigen-presenting cells, NK cells and CD4 + and CD8 + T cells. IFNis a crucial immune component that results from Brucell...

Three culture media (Brucella agar, Farrell medium, and CITA) were compared for their effectiveness in inhibiting contamination and for isolating Brucella spp. One hundred lymph nodes from pigs (n = 50) and wild boars (n = 50) with lymphadenitis were collected in slaughterhouses in the State of São Paulo and were assessed on these three selective media for Brucella spp. All of the samples were ...

Brucella is a facultative intracellular pathogen, and brucellosis is commonest zoonotic disease worldwide. Brucella species, isolated from domestic animals, are important pathogen for humans. Annually, more than 500,000 new cases of brucellosis are reported, and this figure is an underestimate due to extended under-reporting cases in several endemic countries. Brucella has a variety of virulenc...

Seventeen striped dolphins (Stenella coeruleoalba) displaying swimming disorders compatible with neurological syndromes were investigated for Brucella infection. Sixteen dolphins had meningoencephalomyelitis. Serum antibody against Brucella antigen was detected in all 14 animals tested and Brucella ceti was isolated from eight out of nine animals. Brucella antigen was detected in the brain by i...

Brucella contaminates people with raw milk, raw dairy products and especially with cheese made from raw milk. This study has aimed to detect Brucella abortus and Brucella melitensis in fresh cheese (Coban Cheese, 100-mold), traditional Afyon Tulum Cheese (Aged Skin Bag Cheese) (50-mold) and sheep-lamb tulum (50-mold) samples provided from Afyonkarahisar province. Conventional Culture Method has...

Brucellosis is a common infection of feral swine throughout the United States. With the recent expansion of feral swine populations across the country, this disease poses an increasing threat to agriculture and hunters. The standard approach to Brucella surveillance in feral swine has been serological testing, which gives an indication of past exposure and is a rapid method of determining popul...

The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but not reactive in the middle or late stage, and was reactive to Brucella-positive mouse serum in the late stage, but not in the early or middle stage of infection. In addition, rMDH did not react...

brucella is a facultative intracellular pathogen, and brucellosis is commonest zoonotic disease worldwide. brucella species, isolated from domestic animals, are important pathogen for humans. annually, more than 500,000 new cases of brucellosis are reported, and this figure is an underestimate due to extended under-reporting cases in several endemic countries. brucella has a variety of virulenc...