Rifampin is one of the most potent and broad-spectrum antibiotics against bacterial pathogens. Its bactericidal activity is due to its ability to bind to the beta subunit of the DNA-dependent RNA polymerase encoded by the rpoB gene. Mutations of the rpoB gene have been characterized in rifampin-resistant (Rif(r)) strains of Escherichia coli and Mycobacterium tuberculosis. The genetic bases of R...

Although the etiological agent of brucellosis has been known for many years, this widespread infection remains one of the greatest enigmas in modern medicine. Perhaps the principal source of confusion is the difficulty of establishing a definite diagnosis of brucellosis. This is particularly true of the chronic form of this disease. While the history, clinical picture, laboratory procedures, in...

A basic feature of the host-parasite relationship in brucellosis is the intracellular localization of Brucella in the tissues of animals and man (1-6). This intracellular parasitism is a contributing factor in the tendency of the disease to become chronic. Experimental studies have shown that intracellular Brucella organisms are afforded protection against antibiotics, and also against the leth...

Virulent and attenuated Brucella abortus strains attach to and penetrate nonprofessional phagocytic HeLa cells. Compared to pathogenic Brucella, the attenuated strain 19 hardly replicates within cells. The majority of the strain 19 bacteria colocalized with the lysosome marker cathepsin D, suggesting that Brucella-containing phagosomes had fused with lysosomes, in which they may have degraded. ...

The patterns of susceptibility to hydrophobic and hydrophilic drugs and the uptake of the fluorescent probe N-phenyl-naphthylamine in Brucella spp., Haemophilus influenzae, Escherichia coli, and deep rough Salmonella minnesota mutants were compared. The results show that the outer membranes of smooth and naturally rough Brucella spp. do not represent barriers to hydrophobic permeants and that t...

Brucella endocarditis, despite its high mortality rate with combined medical and surgical treatment, has a low occurrence rate in cases of brucellosis and has been endemic in regions surrounding Turkey. Rarely, patients with infective endocarditis with common microorganisms develop a splenic abscess. A patient is reported on with brucella endocarditis and persistent fever. An occult splenic abs...

INTRODUCTION In previous articles the distribution and diversity of Brucella sp infections has been covered. In addition the evasive mechanisms used by Brucella spp in avoiding the innate and acquired immune systems has been discussed in detail. This article will cover diagnostic tests and recent work on Brucella testing and the possible implications in strategies for diagnosis and control. The...

Quantitative cross precipitation studies with goat antisera show the three endoantigens of the brucella to be serologically distinguishable. Although the endoantigens of Br. abortus and Br. suis are very similar, they do not react identically, permitting the serological distinction of the two organisms. These differences in cross precipitation may be used to identify an organism of the brucella...

background: brucellosis is one of the most common zoonosis in middle east and iran. objectives: the purpose of this study was genomic detection of brucella spp. in sero-positive dairy cattle. methods: we have collected 28,519 blood samples from cows during 2012-2013. samples were screened by slide and tube agglutination and 2-mercaptoethanol tests. samples with anti-brucella antibodies titer ≥ ...