In vitro studies on cancer cell lines still constitutes a major part of cancer research. It is very important that the researchers consider the importance of basic conditions of working with different cell lines. To the best of authors knowledge, this study is the first one to report the effect of seeding density on the growth curve of three human cancer cell lines. These cell lines include hum...
Microencapsulation technology is an alternative large-scale mammalian cell culture method. The semi-permeable membrane of the microcapsule allows free diffusion of nutrients, oxygen and toxic metabolites to support cell growth, and the microcapsule membrane can protect the cells from the mechanical damage of shear forces associated with agitation and aeration. Many polymers have been used to ma...
Endothelial cell seeding is a technique that has developed over the past 15 years in response to the need for a high performance synthetic vascular graft. This review details our present knowledge of seeding and examines the various problems that have hampered its introduction into clinical practice.
:Journal of biomedical materials research. Part A2015
Mohammed A Yassin,
Knut N Leknes,
Torbjorn O Pedersen,
Stein A Lie,
Constructs intended for bone tissue engineering (TE) are influenced by the initial cell seeding density. Therefore, the objective of this study was to determine the effect of bone marrow stromal stem cells (BMSCs) density loaded onto copolymer scaffolds on bone regeneration. BMSCs were harvested from rat's bone marrow and cultured in media with or without osteogenic supplements. Cells were seed...
Mohammad Al Hasan,
Mohammed J. Zaki,
The leading partitional clustering technique, k-means, is one of the most computationally efficient clustering methods. However, it produces a local optimal solution that strongly depends on its initial seeds. Bad initial seeds can also cause the splitting or merging of natural clusters even if the clusters are well separated. In this paper, we propose, ROBIN, a novel method for initial seed se...
:Frontiers in bioscience : a journal and virtual library2004
Kristin J Pawlowski,
Stanley E Rittgers,
Steven P Schmidt,
Gary L Bowlin,
Pioneers in the field of small diameter graft development sought to promote graft endothelialization and, thereby, increase patency by transplanting a varying degree of autologous endothelial cells onto vascular grafts prior to implantation. This process has become known as endothelial cell seeding. The underlying hypothesis is quite simple; that is, by promoting the establishment of the patien...
Blair K. Gage,
Travis D. Webber,
Timothy J. Kieffer,
Human embryonic stem cells (hESCs) have the ability to form cells derived from all three germ layers, and as such have received significant attention as a possible source for insulin-secreting pancreatic beta-cells for diabetes treatment. While considerable advances have been made in generating hESC-derived insulin-producing cells, to date in vitro-derived glucose-responsive beta-cells have rem...
:Journal of tissue engineering and regenerative medicine2017
Brian J Huang,
Daniel J Huey,
Jerry C Hu,
Kyriacos A Athanasiou,
Recent work has established methods to engineer self-assembled, scaffold-free neocartilage from an expanded articular chondrocyte (AC) cell source. In continuing such work, the objective of the present study was to investigate the effects of cell-seeding density and dexamethasone concentration on these neocartilage constructs. Neocartilage discs (5 mm diameter) were formed by self-assembling pa...
:Journal of biomedical materials research2000
C E Holy,
M S Shoichet,
J E Davies,
New three-dimensional (3D) scaffolds for bone tissue engineering have been developed throughout which bone cells grow, differentiate, and produce mineralized matrix. In this study, the percentage of cells anchoring to our polymer scaffolds as a function of initial cell seeding density was established; we then investigated bone tissue formation throughout our scaffolds as a function of initial c...