A microarray analysis was performed to investigate whether ex vivo culture conditions affect the characteristics of MSCs. Gene expression profiles were mainly influenced by the level of cell confluence rather than initial seeding density. The analysis showed that 276 genes were upregulated and 230 genes downregulated in MSCs harvested at ~90% versus ~50% confluence (P < 0.05, FC > 2). The genes...

Statement of Purpose: This study was focused on a collagen/cell mixture seeding method to improve the adhesion and proliferation of vascular smooth muscle cells (SMCs) in tubular porous scaffolds for vascular grafts application. Recently, poly(L-lactide-co-εcaprolactone) (PLCL) copolymers have been applied as a biomaterial for vascular graft due to the high elastic property. We previously repor...

Cloned human telomeric DNA can integrate into mammalian chromosomes and seed the formation of new telomeres. This process occurs efficiently in three established human cell lines and in a mouse embryonic stem cell line. The newly seeded telomeres appear to be healed by telomerase. The seeding of new telomeres by cloned telomeric DNA is either undetectable or very inefficient in non-tumourigenic...

BACKGROUND Currently, cationic liposome has become the commonly used vehicles for gene transfection. Furthermore, one of the most significant steps in microRNAs expression studies is transferring microRNAs into cell cultures successfully. In this study we aim to approach the feasibility of transfection of cervical cancer cell lines mediated by liposome and to obtain the optimized transfection c...

"Air-seeding" is a proposed mechanism for the initiation of water stress embolism in dead plant cells. During air-seeding, external air is drawn into the lumen of a dead plant cell through a pore or crack in the cell wall. The resulting bubble may expand to fill the lumen, thus embolizing the cell. The data presented confirm that Sphagnum hyalocysts can embolize by air-seeding when the pressure...

DIFFERENTATIONS *Troken, AJ; +*Mao, JJ (A-NIH) Tissue Engineering Laboratory, University of Illinois at Chicago, Chicago, IL [email protected] INTRODUCTION: Cell density during various stages of the development of synovial joints is generally not well understood. Cell density only accounts for 5% of the total volume of articular cartilage (Stockwell, 1979; Mow et al., 1992; McDevitt et al., 1992). ...

Minimizing computational cost is one of the major challenges in modelling and numerical analysis hydrodynamics, ways to achieve this by use quadtree grids. In paper, we present an adaptive scheme on grids for variable density shallow water equations. A coupled system developed based work [M.A. Ghazizadeh, A. Mohammadian, Kurganov, Computers & Fluids, 208 (2020)]. The capable exactly preserving ...