Adequate cellular in-growth into biomaterials is one of the fundamental requirements of scaffolds used in regenerative medicine. Type I collagen is the most commonly used material for soft tissue engineering, because it is nonimmunogenic and a highly porous network for cellular support can be produced. However, in general, adequate cell in-growth and cell seeding has been suboptimal. In this st...

BACKGROUND The main cause of death in medulloblastoma is recurrence associated with leptomeningeal dissemination. During this process, the role of microRNAs (miRs) in the acquisition of metastatic phenotype remains poorly understood. This study aimed to identify the miR involved in leptomeningeal dissemination and to elucidate its biological functional mechanisms. MATERIALS AND METHODS We ana...

The purpose of this study was to evaluate the behaviors of rat tooth germ (TG) cells cultured on poly(vinyl alcohol) (PVA). It was found that TG cells suspended and aggregated to form three-dimensional spheroids on PVA. Compared with traditional monolayered cells on tissue culture polystyrene, TG cell spheroids on PVA obviously increased the alkaline phosphatase activity, the degree of minerali...

Seeding of distinct intrathymic microenvironments defined by direct thymocyte-stromal cell interactions was correlated with T cell development in situ using radiation and nonradiation chimeras of Thy-1.1/1.2 congenic mice. The results identify associations of thymocytes with I-A- macrophages in the cortex as the earliest discernible cell-cell interactions during thymopoiesis. After a significan...

It is important to predict which invasive species will benefit from future changes in climate, and thereby identify those invaders that need particular attention and prioritization of management efforts. Because establishment, persistence, and spread determine invasion success, this prediction requires detailed demographic information. Explicit study of the impact of pattern on demographic resp...

Using an electrospinning technique, we constructed composite nanofibrous membranes containing a copolymer of L-lactide and glycolide (PLGA, ratio 85:15) and 33% wt.% of nanodiamond particles. The number of initially adhering human osteoblast-like MG 63 cells on day 1 after seeding, their spreading and subsequent growth were similar on both types of membranes. However, higher cell numbers on day...

Generation of human cardiomyocytes (CMs), cardiac fibroblasts (CFs), and endothelial cells (ECs) from induced pluripotent stem (iPSCs) has provided a unique opportunity to study the complex interplay among different cardiovascular cell types that drives tissue development disease. In area models, several sophisticated three-dimensional (3D) approaches use cell-derived (iPSC-CMs) mimic physiolog...

OBJECTIVE Previous work demonstrated the effectiveness of autologous adipose-derived stem cells (ASCs) as endothelial cell (EC) substitutes in vascular tissue engineering. We further this work toward clinical translation by evaluating ASC function after (1) replacement of fetal bovine serum (FBS) with autologous human plasma (HP) in culture and (2) cryopreservation. METHODS Human ASCs and pla...

Ecologists are searching for models, frameworks, and principles that provide a bridge between theory and the practice of restoration. Successional management has been proposed as a useful model for managing and restoring invasive-plant-dominated rangeland because it provides a framework in which ecological processes can be manipulated by managers to achieve a desired plant community. Succession...