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نتایج جستجو برای Conditioned medium

تعداد نتایج: 179077  
Journal: :Journal of visualized experiments : JoVE 2015
Jordi Caballé-Serrano, Kosaku Sawada, Guenther Schuldt Filho, Dieter D Bosshardt, Daniel Buser, Reinhard Gruber,

Autologous bone grafts are widely used in oral and maxillofacial surgery, orthopedics, and traumatology. Autologous bone grafts not only replace missing bone, they also support the complex process of bone regeneration. This favorable behavior of autografts is attributed to the three characteristics: osteoconductivity, osteogenicity, and osteoinductivity. However, there is another aspect: Bone g...

Journal: :Cancer research 2000
D Huang, J L Rutkowski, G M Brodeur, P M Chou, J L Kwiatkowski, A Babbo, S L Cohn,

Neuroblastomas are biologically heterogeneous tumors that consist of two main cell populations: neuroblastic/ganglionic cells and Schwann cells. The amount of Schwannian stroma strongly impacts prognosis, and favorable outcome is associated with tumors that are Schwannian stroma rich/stroma dominant. At the present time, there is controversy regarding the origin of Schwann cells in neuroblastom...

Background & Aims: One of cell- based technical issues associated with cartilage repair assay is delivering cells to the site of the parts where damage is created. Mesenchymal stem cells (MSCs) with their chondrogenic potential are ideal candidates for cartilage regeneration. High expression of cartilage hypertrophy markers by MSCs would result in apoptosis and ossification. This investigation ...

Journal: :Journal of dental research 2002
H Arzate, L F Jiménez-García, M A Alvarez-Pérez, A Landa, I Bar-Kana, S Pitaru,

Little is known about the molecular mechanisms that regulate the cementogenesis process, because specific cementum markers are not yet available. To investigate whether a cementoblastoma-conditioned medium-derived protein (CP) could be useful as a cementum biological marker, we studied its expression and distribution in human periodontal tissues, human periodontal ligament, alveolar bone, and c...

Journal: :Reproductive biology 2011
Malgorzata Duda, Malgorzata Knet, Zbigniew Tabarowski, Maria Slomczynska,

The purpose of the study was to examine the effect of luteal macrophage conditioned medium (LMCM) on progesterone and estradiol production by cultured granulosa cells. Porcine granulosa cells were cultured for 48 h with or without LMCM in the absence or presence of 100 ng/ml LH, FSH or prolactin. Progesterone and estradiol concentrations were measured by radioimmunoassay. Granulosa cells were a...

2014
Paul J Mintz, Kai-Wen Huang, Vikash Reebye, Georgios Nteliopoulos, Hong-Shiee Lai, Pal Sætrom, Noriyuki Kasahara, Steen Jensen, Madhava Pai, Myrtle YA Gordon, Stephen B Marley, Rosemary Behan, Duncan R Spalding, Abdelali Haoudi, Mohamed M Emara, Joanna Nicholls, John J Rossi, Nagy A Habib,

Despite the progress in our understanding of genes essential for stem cell regulation and development, little is known about the factors secreted by stem cells and their effect on tissue regeneration. In particular, the factors secreted by human CD34+ cells remain to be elucidated. We have approached this challenge by performing a cytokine/growth factor microarray analysis of secreted soluble f...

2006
Janet L. Kwiatkowski, J. Lynn Rutkowski, Darrell J. Yamashiro, Gihan I. Tennekoon, Garrett M. Brodeur,

Neuroblastomas arc histopathologically heterogeneous, ranging from immature malignant tumors to benign ganglioneuromas. The amount of Schwann cell stroma greatly increases with neuroblastoma differentiation, and these Schwann cells appear to be normal cells that infiltrate the tumor. To determine whether Schwann cells influence neuroblast differ entiation, four human neuroblastoma cell lines we...

Journal: :Molecular vision 1997
G M Seigel, L Liu,

PURPOSE Apoptosis is implicated in the death of retinal cells during both retinal differentiation and degeneration. We sought to investigate potential diffusible retinal cell signalling factors which may be responsible for this phenomenon. METHODS 72 hr conditioned medium was collected from death-induced R28 retinal cells undergoing serum-starvation. This conditioned medium was filtered, titr...

Journal: :Human reproduction 2010
C M Zumoffen, A M Caille, M J Munuce, M O Cabada, S A Ghersevich,

BACKGROUND Spermatozoa acquire the ability to fertilize an oocyte when they become capacitated. Capacitation takes place when sperm pass through the female reproductive tract, interacting with female fluids. Both tyrosine phosphorylation of sperm proteins and the ability to respond to acrosome reaction (AR) inducers have been associated with sperm capacitation. Recent data indicate that conditi...

Journal: :Experimental hematology 1982
T Hanada, T Nagasawa, T Abe,

To investigate the effects of the conditioned medium on regulation of hematopoietic stem cells, serial volumes of monocyte-conditioned medium were added to the culture systems of CFUE and CFUC. Small volumes of the conditioned medium possessed stimulatory activity and large volumes inhibitory activity for both CFUE and CFUC. These findings suggest that the monocyte-conditioned medium regulates ...