The interaction of four long-chain nicotinates, compounds that are of interest as potential chemopreventive agents, with dipalmitoylphosphatidylcholine (DPPC) was investigated in monolayers at the air-water interface and in fully hydrated bilayers. For the monolayer studies, the compression isotherms of mixtures of the respective nicotinate with DPPC were recorded at various compositions on a h...

Advancing and receding contact angles of water, formamide and diiodomethane were measured on 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) layers deposited on three different solid supports-glass, mica and poly(methyl methacrylate). Up to five statistical monolayers were deposited on the surfaces by spreading DPPC solution. It was found that even on five statistical DPPC monolayers, the hy...

We use (2)H-NMR, (1)H-MAS NMR, and fluorescence microscopy to detect immiscibility in three particular phospholipid ratios mixed with 30% cholesterol: 2:1 DOPC/DPPC, 1:1 DOPC/DPPC, and 1:2 DOPC/DPPC. Large-scale (>>160 nm) phase separation into liquid-ordered (L(o)) and liquid-crystalline (L(alpha)) phases is observed by both NMR and fluorescence microscopy. By fitting superimposed (2)H-NMR spe...

2-(2,4-Dihydroxyphenylo)-5,6-dichlorobenzthiazole (dHBBT) is a new drug from the group of chemical compounds characterized by documented antifungal, antibacterial, cytostatic as well as antitumour activity. Despite general knowledge regarding pharmacological importance of dHBBT its interaction to biomembranes has not been investigated. In this work, we present the electronic absorption spectros...

Wide angle x-ray scattering (WAXS) from oriented lipid multilayers was used to study the effect of adding cholesterol (Chol) or 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) to gel-phase 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) bilayers. Small quantities (X < 0.10 mole fraction) of both molecules disrupt the tight packing of tilted chains of pure gel-phase DPPC, forming a more disor...

The interaction between PAMAM (polyamidoamine) dendrimer generation 4 (G4) and 3,5 (G3,5) with model lipid membranes composed of dipalmytoylphosphatidylcholine (DPPC) has been investigated. Differential scanning calorimetry (DSC) and Raman spectroscopy were applied to assess the thermodynamic changes caused by PAMAM G4 and G3,5 and to specify the exact location of these dendrimers into the DPPC...

The most important component of pulmonary surfactant is dipalmitoyl phosphatidylcholine, or DPPC. At the end of an expiration, when the surfactant film is maximally compressed, DPPC is probably almost the sole component in the monomolecular film, but, during inspiration, as the film expands, other elements move in. They may be phospholipids with different fatty acids, saturated or unsaturated, ...

Pulmonary surfactant is a complex mixture, but clearly has one main component, dipalmitoylphosphatidylcholine, or DPPC. The respiratory distress syndrome, RDS, a condition afflicting the baby born too early, is caused by a deficiency of pulmonary surfactant. When this was first realized, attempts were made to treat the condition by supplying DPPC, the main component of the missing surfactant. T...

Lung surfactant dipalmitoylphosphatidylcholine (DPPC) is endocytosed by alveolar epithelial cells and degraded by lysosomal-type phospholipase A2 (aiPLA2). This enzyme is identical to peroxiredoxin 6 (Prdx6), a bifunctional protein with PLA2 and GSH peroxidase activities. Lung phospholipid was studied in Prdx6 knockout (Prdx6-/-) mice. The normalized content of total phospholipid, phosphatidylc...

BACKGROUND Nanosuspensions are an important class of delivery system for vaccine adjuvants and drugs. Previously, we developed a nanosuspension consisting of the synthetic TLR4 ligand glucopyranosyl lipid adjuvant (GLA) and dipalmitoyl phosphatidylcholine (DPPC). This nanosuspension is a clinical vaccine adjuvant known as GLA-AF. We examined the effects of DPPC supplier, buffer composition, and...