We report here that polyethylene glycol (PEG) linked to near infrared dyes conjugated to chimeric mouse-human anti-carcinoembryonic antigen (CEA) antibody greatly improves imaging of liver metastases in a nude mouse model of colon-cancer experimental metastases. PEGylated and non-PEGylated DyLight 650 and 750 dyes were conjugated to the chimeric anti-CEA antibody. The dyes were initially inject...

Covalent derivatization of proteins with fluorescent dyes prior to separation is increasingly used in proteomic research. This paper examines the properties of several commercially available iodoacetamide and maleimide dyes and discusses the conditions and caveats for their use in labeling of proteomic samples. The iodoacetamide dyes BODIPY TMR cadaverine IA and BODIPY Fl C(1)-IA were highly sp...

Fluorescence microscopy of cells loaded with fluorescent, Ca2+-sensitive dyes is used for measurement of spatial and temporal aspects of Ca2+ signaling in live cells. Here we describe the method used in our laboratories for loading suspensions of human sperm with Ca2+-reporting dyes and measuring the fluorescence signal during physiological stimulation. Motile cells are isolated by direct swim-...

Fluorescent nucleic acid hybridization probes traditionally have been generated by enzymatic incorporation of dye-labeled nucleotides, even though incorporation efficiency is low and variable from dye to dye. Alternatively, 5-(3-aminoallyl)-2'-deoxyuridine 5'-triphosphate (aa-dUTP) is enzymatically incorporated to generate amine-modified DNA, which is then chemically labeled with an amine-react...

Cy3 and Cy5 are among the most commonly used oligonucleotide labeling molecules. Studies of nucleic acid structure and dynamics use these dyes, and they are ubiquitous in microarray experiments. They are sensitive to their environment and have higher quantum yield when bound to DNA. The fluorescent intensity of terminal cyanine dyes is also known to be significantly dependent on the base sequen...

the used activated carbon was derived from pine-cone and was used for the removal of acid black 1(ab1) and acid blue 113(ab113) dyes. a batch sorption study was carried out in order to obtain optimum isotherm model. the study showed the best fit of the adsorption isotherm data was obtained using the langmuir model for both dyes. the monolayer maximum saturation capacities of ab1 and ab113 dyes ...

This study compares the performance of simulated shallow ponds vegetated with Lemna minor L. under controlled and semi-natural conditions for the treatment of simulated wastewater containing textile dyes. The objectives were to assess the water quality outflow parameters, the potential of L. minor concerning the removal of chemical oxygen demand (COD) and four azo dyes (Acid blue 113, reactive ...

Natural dyes are widely used in the coloring of food, leather and wood [1]. Traditionally they were the only colorants for textile fibers like wool, silk, cotton and flax. Their use in textiles declined drastically with the introduction of synthetic dyes in 1856 [2-6]. Presently the use of synthetic dyes is estimated at around 10,000,000 tons per annum [7-11]. The manufacture and use of this la...

Azo dyes are one of the oldest industrially synthesized organic compounds characterized by presence of Azo bond (-N=N-) and are widely utilized as coloring agents in textile, leather, cosmetic, paint, plastic, paper, and food industries During textile processing, inefficiencies in dyeing result in large amounts of the dyestuff (varying from 2% loss when using basic dyes to a 50% loss when certa...