Loss of (genomic) imprinting (LOI) of the insulin-like growth factor 2 gene (IGF2) is a common epigenetic abnormality in many human cancers. IGF2 imprinting is regulated by differentially methylated domains (DMD) in the imprinting control region that is located between IGF2 and H19 on human chromosome 11. In the present study, combined expression of adenoviral vectors (Ad-EGFP and Ad-E1A) drive...

Our previous study demonstrated that PTB-associated splicing factor (PSF) is an important regulator of cell death and plays critical roles in the survival and growth of colon cancer cells. However, the molecular mechanism that activates these downstream signaling events remains unknown. To address this issue, we investigated the effects of PSF knockdown in two different colon cancer cell lines,...

AIM To explore the effects of PCK3145 beyond prostate cancer. MATERIALS AND METHODS Using Trypan blue, MTT proliferation assays, cell cycle and apoptosis analysis, we assessed the effects of PCK3145 on prostate (PC-3), breast (MCF-7) and colon (HT-29) human cancer cell lines and in osteosarcoma (MG-63) cells; any synergistic effects with docetaxel and oxaliplatin were also explored. RESULTS...

Curcumin, a major yellow pigment and active component of turmeric, has been shown to possess anti-inflammatory and anticancer activities. Cyclooxygenase (COX)-2 plays an important role in colon carcinogenesis. To investigate the effect of curcumin on COX-2 expression, we treated HT-29 human colon cancer cells with various concentrations of curcumin. Curcumin inhibited the cell growth of HT-29 c...

AIM To investigate the effect of paeonol on controlling the proliferation of colorectal cancer cell line HT-29 and to discuss its possible mechanism. METHODS The inhibitory effect of paeonol on proliferation of HT-29 cells was detected by MTT assay. The results of apoptosis were measured by flow cytometry. Immunocytochemical staining was performed to detect the expression of cyclooxygenase-2 ...

Liver metastasis is the most frequent cause of death from colon and other cancers. Generally, liver metastasis is recalcitrant to treatment. The aim of this study is to determine the efficacy of tumor-targeting Salmonella typhimurium A1-R on liver metastasis in orthotopic mouse models. HT-29 human colon cancer cells expressing red fluorescent protein (RFP) were used in the present study. S. typ...

Abstract Background Clerodendrum is a genus of about 500 species belongs to the family Lamiaceae. Many this have been proved for treatment various diseases. This study was aimed evaluate cytotoxic effect different solvents and their most active fractions thomsoniae Balf.f. in human cancer cell lines. Aerial parts plant were subjected Soxhlet extraction. Phytochemical analysis done by using stan...

introduction: neurotensin (nt) is a tridecapeptide that binds specifically to neurotensin receptors. several forms of cancer, including small cell lung cancer, colon, pancreatic and prostate carcinomas especially exocrine pancreatic carcinomas express receptors for neurotensin peptide. radiolabeled neurotensin derivatives with a high affinity for these receptors might be used for scintigraphy. ...

Phosphodiesterase is an enzyme that degrades the phosphodiester bond in second messenger molecules cAMP and cGMP, regulates localization, duration, amplitude of cyclic nucleotide signaling within subcellular domains. Sildenafil a potent selective inhibitor type 5 cGMP-specific phosphodiesterase used clinically to treat erectile dysfunction pulmonary arterial hypertension. In this study, we exam...