Highly sensitive techniques are needed for the quantitative determination of mitochondrial oxidative phosphorylation function in single rat hippocampal slices or isolated hippocampal subfields. We determined the oxygen consumption of single hippocampal slices or subfields applying high-resolution respirometry adapted for slice measurements and measured the redox state of mitochondrial NAD(P)H i...

introduction: the hippocampus was recognized as an important focus of epileptic seizures. a long-term potentiation (ltp) like that of epileptiform activities is considered as synaptic which has been observed for the first time in hippocampal formation. the purpose of the present study was investigating the synaptic plasticity induced by tetanic stimulation at ca1 area of the rat hippocampal sli...

of Dissertation Presented to the Graduate School of the University of Florida in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy DIFFUSION MAGNETIC RESONANCE IMAGING IN A RAT HIPPOCAMPAL SLICE MODEL OF ACUTE BRAIN INJURY By Timothy Michael Shepherd May 2003 Chair: Edward D. Wirth, III Major Department: Neuroscience This dissertation describes experiments using rat...

the effect of endogenous serotonin on long-term potentiation (ltp) in region cai was studied by blocking 5-ht2 receptors with ketanserin in rat hippocampal slices. such a block significantly en-hanced long-term potentiation of the cai population spike induced by high frequency stimulation of the schaffer collateral/ commissural pathway. this implies that serotonin acts on 5-ht2 receptors in cai...

objective(s):to determine the effect of acetylcholine (ach), pilocarpine, and atropine on pain evoked responses of pain excited neurons (pen) and pain inhibited neurons (pin) in hippocampal ca3 region of morphine addicted rats. materials and methods:female wistar rats, weighing between 230-260 g were used in this study. morphine addicted rats were generated by subcutaneous injection of increasi...

Corticotropin-releasing factor (CRF) exerts a key neuroregulatory control on stress responses in various regions of the mammalian brain, including the hippocampus. Using hippocampal slices, extracts, and whole animals, we investigated the effects of human/rat CRF (h/rCRF) on hippocampal neuronal excitability and hippocampus-dependent learning in two mouse inbred strains, BALB/c and C57BL/6N. In...

Virus-mediated gene transfer into neurons is a powerful tool for the analysis of neuronal structure and function. Recombinant sindbis virus has been previously used to study protein function in hippocampal neuron cultures as well as in hippocampal organotypic slice cultures. Nevertheless, some concern still exists about the physiological relevance of these cultured preparations. Acute hippocamp...

Rett syndrome is an autism-spectrum disorder caused by loss of function mutations within the gene encoding methyl CpG-binding protein 2 (MeCP2). While subtle decreases in synaptic plasticity have been detected within cortical and hippocampal neurons of Mecp2-null mice, only minimal information exists regarding how the loss of MeCP2 affects network activity in the brain. To address this issue, w...

Incubation of the acutely dissected rat hippocampal slices in calcium-containing media resulted in spontaneous activation-translocation of classical PKC isoforms and their subsequent (especially gamma-type) proteolytic degradation. These changes were blocked by calpain inhibitor MDL 28 170 in 100 microM concentration. Rat hippocampal slices were metabolically prelabelled with 32Pi and stimulate...