A method of determining histidine decarboxylase activity was established. In this method, 14C-histamine was separated from 14C-histidine by Amberlite CG-resin column in the ammonium form. Lecanoric acid was obtained by screening histidinedecarboxylase inhibitors produced by microorganisms. It is the first isolation of this compound from fungi. The inhibition by lecanoric acid was competitive wi...

im-Dinitrophenyl histidine does not decompose under the standard conditions of protein hydrolysis, and it emerges between phenylalanine and lysine on the short column of the amino acid analyzer. Its ninhydrin color constant was found to be 76% of that for histidine. A method for analysis of im-dinitrophenyl histidine in a reacted protein is described. I-Fluoro-2,4-dinitrobenzene reacts preferen...

To investigate the cause of low serum histidine in rheumatoid arthritis (RA) single oral and intravenous doses of L-histidine were administered to patients with active RA, and to an equal number of age and sex matched control subjects. In the first study 13 patients and their controls received a 100 mg kg-1 dose of L-histidine as an aqueous slurry. Significant differences were seen in body weig...

In this work, we designed and synthesized a heterobimetallic ruthenium(ii)-nickel(ii) complex, [Ru(bpy)2(phen-DPA)Ni](PF6)4 (Ru-Ni), as a highly selective phosphorescence probe for histidine. The probe exhibited weak emission at 603 nm because the phosphorescence of the Ru(ii) complex can be strongly quenched by the paramagnetic Ni(2+) ion. In the presence of histidine, reaction of Ru-Ni with h...

Flor yeasts of Saccharomyces cerevisiae have an innate diversity of Flo11p which codes for a highly hydrophobic and anionic cell-wall glycoprotein with a fundamental role in biofilm formation. In this study, 380 nitrogen compounds were administered to three S. cerevisiae flor strains handling Flo11p alleles with different expression levels. S. cerevisiae strain S288c was used as the reference s...

The genetic control of histidine biosynthesis in Staphylococcus aureus was examined by an analysis of 66 histidine-dependent mutants by using stable and abortive transduction. These mutants, which previously had been differentiated into classes according to their biochemical characteristics, all occupied sites within a single linkage group, referred to as the histidine region. This region has b...

common to both these compounds (Heath & Wildy, 1956a, b). These results indicated that this fungus forms ergothioneine by methylation and sulphuration of histidine. Subsequent work (Wildy & Heath, 1956, 1957) revealed that [2(ring)-14C]histamine would not serve as a precursor of the imidazole nucleus of either histidine or ergothioneine. Various metabolites of histamine, imidazoles possessing m...

The effect of lead on the release of [14C]GABA, [3H]dopamine and [14C]histidine (as a precursor of histamine) was studied in synaptosomes obtained from chronically lead-treated rats and in synaptosomes with in vitro lead added. In vivo treatment of rats with lead acetate results in a decrease in the K(+)-depolarization-dependent release of GABA and dopamine and histidine. Lead given in vitro it...

Histidine is a convenient tridentate chelator used in the synthesis of technetium-99m radiopharmaceuticals, as it can be pendantly attached to a biomolecule for molecular imaging applications. Once coordinated, it forms a neutral complex that is capable of forming diastereomers at the alpha amine of the histidine. This is demonstrated through the synthesis and characterization of four different...

Transient network hydrogels cross-linked through histidine-divalent cation coordination bonds were studied by conventional rheologic methods using histidine-modified star poly(ethylene glycol) (PEG) polymers. These materials were inspired by the mussel, which is thought to use histidine-metal coordination bonds to impart self-healing properties in the mussel byssal thread. Hydrogel viscoelastic...