:Applied and environmental microbiology1997
A L Henderson,
T C Schmitt,
T M Heinze,
C E Cerniglia,
Intestinal microfloras from human, rat, mouse, and monkey fecal samples and 14 pure cultures of anaerobic bacteria representative of those found in the human gastrointestinal tract metabolized the triphenylmethane dye malachite green to leucomalachite green. The reduction of malachite green to the leuco derivative suggests that intestinal microflora could play an important role in the metabolic...
This paper describes a reserved-phase high-performance liquid chromatographic (HPLC) method for detecting malachite green (MG) and leuco-malachite green (LMG) using an isocratic toxic organic solvent/reagent-free mobile phase. Chromatographic separations were performed an Inertsil WP300 C4 with 0.02 mol/L octane sulfonic acid–ethanol mobile phase and a photodiode-array detector. The total run t...
The classical malachite green (MLG) assay of phosphate, which added MLG after molybdate to the acidified reaction solutions of phosphate, tolerated interference from papaverine, sildenafil, and some similar hydrophobic amines. Resonance Rayleigh scattering signals, the alleviation of interference by poly(vinyl alcohol), and the precipitation of some yellow complexes supported that the irreversi...
T P Geladopoulos,
T G Sotiroudis,
A E Evangelopoulos,
A simple and sensitive colorimetric assay for protein phosphatase activity based on the determination of released Pi by an improved malachite green procedure (A. A. Baykov, O. A. Evtushenko, and S.M. Avaeva, 1988, Anal. Biochem. 171, 266-270) is described. Proteins must be removed or stabilized prior to Pi determination with 0.25 N sulfuric acid or 3% (w/w) perchloric acid. Alternatively, to av...
George A Kraus,
Ahmed M Awad,
A series of fluorinated analogs of malachite green (MG) have been synthesized and their toxicity to Saccharomyces cerevisiae and a human ovarian epithelial cell line examined. The toxicity profiles were found to be different for these two species. Two analogs, one with 2,4-difluoro substitution and the other with 2-fluoro substitution seem to be the most promising analogs because they showed th...
Preparations are prepared, using completely clean slides and aqueous suspensions of young bacterial agar cultures. A drop of the suspension is placed on one end of the slide; the slide is then tipped, making the suspension drain down to the opposite end (Society of American Bacteriologists, Manual of Microbiological Methods, McGraw-Hill Book Co., New York, 1957). Both the mordanting and the sta...
P. Antony Soloman,
C. Ahmed Basha,
This study involves the electro-oxidation of malachite green, a triphenyl methane dye, extensively used in industries and aquaculture, and later banned in most developed countries because of its potential carcinogenicity, mutagenicity and teratogenicity in mammals. The study is conducted in a batch electro-chemical reactor using the catalytic anode (made of noble oxide coated, RuOx-TiOx, titani...
The organic dyes directly pollute the soil, water, plants and all living systems in the environment. In the present investigation, adsorption of brilliant green dye on silica from aqueous solution was studied. The dye adsorption mechanism was studied on the basic surface of silica. The adsorption parameters were optimized to maximum adsorption. The adsorption mechanism of brilliant green on sil...