Bottom up assembly of functional molecular ensembles with novel properties emerging from composition and arrangement of its constituents is a prime goal of nanotechnology. By single-molecule cut-and-paste we assembled binding sites for malachite green in a molecule-by-molecule assembly process from the two halves of a split aptamer. We show that only a perfectly joined binding site immobilizes ...

The biological function of specific gene products often is determined experimentally by blocking their expression in an organism and observing the resulting phenotype. Chromophore-assisted laser inactivation using malachite green (MG)-tagged antibodies makes it possible to inactivate target proteins in a highly restricted manner, probing their temporally and spatially resolved functions. In thi...

Pollution of water resources by various pollutants is nowadays a global environmental issue. Among the different type of water pollutants, dye represents a major polluting group. In this study, Azolla filiculides roots have been used for adsorption of Malachite Green (MG), a cationic dyed from aqueous solution. In this study, effects of operating parameters such as initial concentration of dye ...

We reinterpret the scanning-probe Raman spectra shown in the above paper [C.C. Neacsu et al., Phys. Rev. B 73, 193406 (2006)] and compare it to a variety of single-molecule surface-enhanced Raman (SER) studies. The observed blinking behaviour and spectral features must be attributed to carbon contaminations rather than to Malachite Green (MG) single molecules, because, under the given experimen...

Seven-thousand-and-thirty-six specimens of faeces were examined for Salmonellae using three enrichment culture media in parallel.Rappaport's magnesium chloride malachite green broth medium proved superior to selenite F and tetrathionate broths in the recovery of a wide variety of Salmonella serotypes. However, it was inferior to selenite broth in the recovery of Salmonella typhi.A higher recove...

There exist many methods for the application of the Gram stain to histological sections, and with all of them there is generally no difficulty in recognising Gram-positive organisms. However, with most of the well-recognised methods Gram-negative bacteria are difficult to distinguish from the background. Twort's method distinguishes Gram-negative bacteria from the background but is relatively t...

conclusions the present research demonstrated that the ultrasonic (us) process can be utilized as an effective and simple method for treatment of mg dye in an aqueous solution. moreover, the eeo is directly proportional to the mg concentration and power density and inversely proportional to temperature and mechanical agitation, which can be predicted by the obtained empirical kinetics model. co...

A series of fluorinated analogs of malachite green (MG) have been synthesized and their toxicity to Saccharomyces cerevisiae and a human ovarian epithelial cell line examined. The toxicity profiles were found to be different for these two species. Two analogs, one with 2,4-difluoro substitution and the other with 2-fluoro substitution seem to be the most promising analogs because they showed th...