The objective of this study was to determine the effect of different concentrations of anolyte on saprolegniasis in comparison with green malachite in rainbow trout hatcheries, in the northern part of Iran, Tonekabon. Nearly 5000 green eggs of Oncorhynchus mykiss (equivalent to 300 g) were obtained from a private farm in the north of Iran. The study was designed in 5 treatments, 0.25, 0.5, 30 a...

Embryonic mesenchyme limb (LB) and midbrain (CNS) cells cultured at high density were exposed to different concentrations of the fungicide malachite green (MG) and its major metabolite leucomalachite green (LMG). The concentration response curves were analysed, and two endpoints were calculated: the concentrations (μg/mL) that produced 50% inhibition of viability/proliferation (IC50-P) and diff...

Femtosecond time-resolved infrared spectroscopy was employed to study intramolecular charge transfer in triphenylmethane dyes, including malachite green (MG), malachite green carbinol base (MGCB), and leucomalachite green (LMG). A local excited state (LE) and a twisted intramolecular charge-transfer (TICT) state have been observed directly in MG. Furthermore, solvent-controlled TICT measurement...

The organic dyes directly pollute the soil, water, plants and all living systems in the environment. In the present investigation, adsorption of brilliant green dye on silica from aqueous solution was studied. The dye adsorption mechanism was studied on the basic surface of silica. The adsorption parameters were optimized to maximum adsorption. The adsorption mechanism of brilliant green on sil...

Enzymes Immobilization onto different types of the substrate could be helpful in various applicationsof biomedical devices and biosensors. Enzyme activity and stability could be affected by support andmethod of immobilization. In this study, the silver metal foam was successfully synthesized by thesoft-shell method and then was coated with silica. Then, glucose oxidase (GOx) immobilized on nonc...

Biodegradation and detoxification of dyes, Malachite green, Nigrosin and Basic fuchsin have been carried out using two fungal isolates Aspergillus niger, and Phanerochaete chrysosporium, isolated from dye effluent soil. Three methods were selected for biodegradation, viz. agar overlay and liquid media methods; stationary and shaking conditions at 25 °C. Aspergillus niger recorded maximum decolo...

Mycobacteria can tolerate relatively high concentrations of triphenylmethane dyes such as malachite green and methyl violet. To identify mycobacterial genes involved in the decolorization of malachite green, a transposon mutant library of Mycobacterium smegmatis mc2 155 was screened for mutants unable to decolorize this dye. One of the genes identified was MSMEG_5126, an orthologue of Mycobacte...

in this study, rainbow trout (oncorhynchus mykiss) eggs were treated with effective doses of  daenensis thyme, thymus daenensis (20, 10 and 5 mgl-1) and mentha, mentha longifolia (10, 5 and 2.5 mgl-1), during incubation period (20 days) until hatching for 30 minutes per every other day as constant flow bath treatment method. the mortality level in normal control (without any treatment) was sign...

OBJECTIVE This study investigated in vitro the photodynamic antimicrobial effects of the photosensitizer malachite green on clinical strains of Staphylococcus, Enterobacteriaceae, and Candida. MATERIALS AND METHODS Thirty-six microbial strains isolated from the oral cavity of patients undergoing prolonged antibiotic therapy, including 12 Staphylococcus, 12 Enterobacteriaceae, and 12 Candida s...

A simple and sensitive colorimetric assay for protein phosphatase activity based on the determination of released Pi by an improved malachite green procedure (A. A. Baykov, O. A. Evtushenko, and S.M. Avaeva, 1988, Anal. Biochem. 171, 266-270) is described. Proteins must be removed or stabilized prior to Pi determination with 0.25 N sulfuric acid or 3% (w/w) perchloric acid. Alternatively, to av...