Introduction: Neuropathic pain may be due to a primary insult to the peripheral or central nervous system. In this situation, Hyperalgesia and Allodynia are the results of prostaglandins and cytokines release in the spinal cord. It seems that immune cells play an importat role in the induction and maintenance of chronic pain. Compared to selective CoxII inhibitors, nimesulide, a highly select...

Microglial cells play a critical role in the neuroinflammatory response that accompanies various diseases of the central nervous system, such as ischemic stroke, and ATP is a major signaling molecule regulating the response of these cells to these pathophysiological conditions. Experiments were carried out to determine the effects of afobazole on microglial function and to identify the molecula...

When the leech central nervous system (CNS) is injured, microglial cells migrate to the site of the lesion. It is possible that the injured CNS releases diffusible substances that alter the properties of microglial cells; to investigate this, microglial cells were cultured in the presence of injured or uninjured CNS tissue. Grown on Concanavalin A (Con-A), 75 % of microglial cells are rounded i...

microglia cells are a subset of central nervous system (cns) macrophages. changes in the cns such as injury, or developmental events, follow by morphological and physiological changes in microglia cells. in this study organotypic brain slice cultures under serum free condition were used to investigate the morphology and lectin histochemistry of microglia and macrophages in the cns in vitro. mic...

The histochemical demonstration of nucleoside diphosphatase as a specific microglial marker was used to study the distribution of this glial cell type in the cerebral cortex of Podarcis muralis and Podarcis hispanica. Our results showed that in both species, NDPase staining was specific for the microglial cell population and that microglial cells displayed a specific localization pattern in the...

Microglial cells within the developing central nervous system (CNS) originate from mesodermic precursors of hematopoietic lineage that enter the nervous parenchyma from the meninges, ventricular space and/or blood stream. Once in the nervous parenchyma, microglial cells increase in number and disperse throughout the CNS; these cells finally differentiate to become fully ramified microglial cell...

To determine whether trophozoites and lysates of pathogenic Acanthamoeba spp. induce apoptosis in primary-culture microglial cells, transmission electron microscopic (TEM) examinations, assessment of DNA fragmentation by agarose gel electrophoresis, and the TdT-mediated dUTP nick-end labeling assay were performed. When a trophozoite of pathogenic Acanthamoeba culbertsoni came in contact with a ...

Jimpy is a genetic disorder which results in a severe hypomyelination in the central nervous system associated with a variety of astroglial and oligodendroglial abnormalities. In this study, we examined the morphology and distribution of microglial cells in spinal cord sections from jimpy and normal mice at 10-12 and 20-22 days postnatal using a specific microglial marker, the nucleoside diphos...

The role of microglia during neurodegeneration remains controversial. We investigated whether microglial cells have a neurotoxic or neuroprotective function in the retina. Retinal explants from 10-day-old mice were treated in vitro with minocycline to inhibit microglial activation, with LPS to increase microglial activation, or with liposomes loaded with clodronate (Lip-Clo) to deplete microgli...

Microglia is considered the only cell population of mesodermal origin, which comprises 10% of the cells in brain parenchyma. Recent neural stem cell (NSC) studies demonstrate that the brain has regenerative potential. NSCs do not give rise to microglial cells, however indicating that NSCs alone cannot complete the regenetion of the brain. Although the role of microglia is not fully understood, ...