Quantification of genetic modification (GM) is often undertaken to test for compliance with the European Union GM labeling threshold in food. Different control laboratories will often use common validated methods, but with different models of real-time PCR machines. We performed two separate ring trials to evaluate the relative precision and accuracy of different types of real-time PCR machines...

Functional studies of resident microglia require molecular tools for their genetic manipulation. Here we show that microRNA-9-regulated lentiviral vectors can be used for the targeted genetic modification of resident microglia in the rodent brain. Using transgenic reporter mice, we demonstrate that murine microglia lack microRNA-9 activity, whereas most other cells in the brain express microRNA...

Dale Pearse School of Agricultural & Resource Economics, University of Western Australia A choice modeling approach was used to identify consumer preferences for various hypothetical forms of genetic modification in beer, using a sample from Western Australia. It was found that respondents were equally averse to first-generation modification in either plants or microorganisms but were willing t...

The Three Rs are the main principles used by Animal Ethics Committees in the governance of animal experimentation, but they appear not to cover some ethical issues that arise today. These include: a) claims that certain species should be exempted on principle from harmful research; b) increased emphasis on enhancing quality of life of research animals; c) research involving genetically modified...

Public opposition to genetic modification (GM) technology in the food domain is widespread (Frewer et al., 2013). In a survey of U.S. residents representative of the population on gender, age, and income, 64% opposed GM, and 71% of GM opponents (45% of the entire sample) were "absolutely" opposed-that is, they agreed that GM should be prohibited no matter the risks and benefits. "Absolutist" op...

the pollution of water environments is a challenging issue especially in developing countries. contamination of drinking water with heavy metals has been reported in many parts of the world. arsenic, chromium and lead are dangerous heavy metals and also common contaminants of drinking water. in this study, the capacity and performance of the surfactant-modified sabzevar natural nanozeolite (sms...

a study on the evaluating equivalent damping and response modification factors of frames equipped by pall friction dampers is presented. to do so, buildings with various stories were considered. nonlinear and linear dynamic time history analysis has been performed to evaluate equivalent damping for pall friction dampers. ground motions with various frequency characteristics scaled with iranian ...

the effects of solvent, temperature, time, weight percent of catalyst on the rate and mechanism of cyclization of natural rubber (nr) was studied in toluene and xylene solutions having tin tetra chloride catalyst (sncl4). iodometric titration show, with 8% sncl4 (based on polymer weight) cyclization occurs, leaving 27.4% of the total unsaturation. infrared spectra of cyclized nr show decreased ...

results the behavioral modification of mothers with the barkley method significantly enhanced their resiliency with children of adhd in the experimental group compared to the control group. patients and methods twenty-four mothers of children diagnosed with adhd were randomly enrolled in this study. the mothers were selected from the 22nd of bahman hospital of qazvin city. mothers with a low sc...