Background: Quantitative fluorescence-polymerase chain reaction (QF-PCR) is an inexpensive and accurate method for the prenatal diagnosis of aneuploidies that applies short tandem repeats (STRs) as a chromosome-specific marker. Despite its apparent advantages, QF-PCR is not applicable in all cases due to the presence of uninformative STRs. This study was carried out to investigate the efficienc...

conclusions this is the first research in comparing two genetic marker systems in p. variotti. we were prompted to explore polymorphisms utility in p. variotti with a look at using germplasm screening mapping of genome and strain improvement programs. results both systems discriminated 20 isolates of p. variotii successfully but were different in the amount of detectable polymorphism. using clu...

background and objectives : dna molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings. materials and methods : here a pcr-supported procedure is described that uses 10 primer pairs targeting chromosomal dna from the harmless vaccinal bacillus anthracis sterne 34f2 strain as template. a single pcr protocol is used to reproduce...

genetic diversity relationships of 50 isolates of cytospora schulzeri on apple from different parts of the semirom region were analyzed using 15 polymerase chain reaction (pcr) based markers, 7 random amplified polymorphic dnas (rapds) and 8 microsatellite primed polymerase chain reaction (mp-pcr). using 7 selected rapd primers 113 bands were generated, of which 81 bands were polymorphic (71.7%...

background: cockroaches are of vital importance medically and hygienically as they can disperse human patho­genic agents and are especially responsible for food contamination and spreading of food borne pathogens. in this study, part of mtdna-coi gene of five common pest cockroaches was tested for diagnostic and phylogenetic pur­poses. methods: we have described barcode region of mtdna-coi gene...

background: equine piroplasmosis is caused by two haemoprotozoan parasites: babesia caballi and theileria equi . negative economic impact on international trade has been associated to endemic sites. this is the reason why carrier detection requires reliable diagnostic methods. various diagnostic modalities can be used alone or in combination including pcr. however, genetic variation of commonly...

background: downy mildew caused by plasmopara halstedii is one of the most devastating diseases of sunflower worldwide. so far several dominant resistance genes designated as pl have been identified and their molecular markers linked to these genes are available. however, no information on the resistance genes is available in iranian lines. objective: in this study, the presence of three map-ba...

the effectiveness of irap, remap, ssr, and issr markers were investigated to assess genetic diversity among and within eight medicago sativa l. populations. a total of 101, 119, 117 loci and 31 alleles were amplified using 10 irap, 14 remap, 16 issr and eight ssr primers, respectively. irap markers generated the maximum proportion of polymorphic loci per primer (pplp) while the maximum value of...