Successful disease management requires a rapid and sensitive diagnosis method that can recognize early infection even before the manifestation of its clinical signs. The only available field diagnostic tests for foot-and-mouth disease (FMD) are lateral flow devices, commonly known as chromatographic strips. Low sensitivity and inability to detect FMD virus (FMDV) at the serotype level are limit...

conclusions the analytical sensitivity of lamp and pcr methods, using the designed primers, was 8 times more than any other reported methods. the designed methods are specific and sensitive for detection of h. pylori in different clinical and environmental samples. results the detection limits of the lamp and pcr assay were similar and were 10 fg of pure dna of h. pylori, which is equal to 6 co...

successful disease management requires a rapid and sensitive diagnosis method that can recognize early infection even before the manifestation of its clinical signs. the only available field diagnostic tests for foot-and-mouth disease (fmd) are lateral flow devices, commonly known as chromatographic strips. low sensitivity and inability to detect fmd virus (fmdv) at the serotype level are limit...

background and objectives: loop-mediated isothermal amplification is a novel nucleic acid amplification assay providing as a simple diagnostic tool for rapid identification of microbial diseases in developing countries. in this study, a lamp assay was established for yersinia enterocolitica , a leading cause of acute enterocolitis in young children. materials and methods: lamp assay was establi...

Bovine viral diarrhea virus (BVDV) is a pathogen that infects cattle, and is globally important. It causes substantial financial losses to the livestock industry. In the current study, a one-step reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay was set up for rapid and efficient detection of BVDV. For this purpose, four primers were designed to recognize six distinct...

A molecular diagnostic method for robust detection of Ebola virus (EBOV) at the point of care (POC) directly from blood samples is described. This assay is based on reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) of the glycoprotein gene of EBOV. Complete reaction formulations were lyophilized in 0.2-mL polymerase chain reaction tubes. RT-LAMP reactions were performed on ...

We developed a one-step, single-tube genogroup-specific reverse transcription–loop-mediated isothermal amplification (RT-LAMP) assay for the detection of group B Coxsackie genomes targeting 5’ UTR region. The amplification can be obtained in less than 1 h by incubating all of the reagents in a single tube with reverse transcriptase and Bst DNA polymerase at 63°C. Detection of gene amplification...