BACKGROUND Allergen measurements are widely used for environmental exposure assessments and for determining the potency of allergen vaccines, yet few purified allergen standards have been developed. The aim of the study was to develop a single standard containing multiple purified allergens that could be used in enzyme immunoassays and in multiplex arrays for the standardization of allergen mea...

Immunoglobulin (Ig) E-mediated sensitization to domestic inhalant allergens (eg, dust mite, cockroach, cat, and dog) is the most important risk factor for asthma. The severity of asthma increases among atopic patients exposed to high levels of sensitizing allergen. In addition, synergism between high allergen exposure and respiratory virus infection increases the risk of asthma exacerbation. Ho...

Allergy diagnosis and immunotherapy in Korea rely mostly on imported allergen extracts. However, some allergens that are not important in Western countries are not commercially available, and even the same species of allergen source often displays differences in allergenicity due to amino acid sequence polymorphisms. Therefore, it is essential to prepare allergen extracts that reflect regional ...

Background The current tools available for diagnosing food allergy include the clinical history, trial elimination diets, diet diaries, skin prick testing (SPT) and allergen-specific serum IgE testing. Since 15 years, development of allergen-based microarrays using recombinant/natural proteins is increasing, resulting in commercially available microarrays to detect specific IgE against more tha...

Correction of an imbalance between allergen-specific T cell subsets is considered a critical event in establishing allergen tolerance by specific immunotherapy (SIT). In a comprehensive, longitudinal study, distinct T cell populations and Ig subtypes were analyzed in subjects allergic to birch pollen during decisive time points of SIT (i.e., induction and maintenance phase), as well as in and o...

AMA Bogacz-Piaseczyńska A, Bożek Pastuszczak M, Zalejska-Fiolka J. Molecular profiling of allergen-antibody IgE might decide about the efficacy allergen immunotherapy in a patient with atopic dermatitis and allergy to house dust mites. Postępy Dermatologii i Alergologii/Advances Dermatology Allergology. 2023. doi:10.5114/ada.2023.129456. APA Bogacz-Piaseczyńska, A., Bożek, Pastuszczak, M., & Za...

The variability of repeated house dust mite (HDM) allergen determinations at the same site within 3-24 months was evaluated on previously collected samples. Between two and four repeated measurements of Der p 1, a major allergen of Dermatophagoides pteronyssinus and Der f 1, a major allergen of D. farinae, on 46 carpets and 31 mattresses were analyzed. In 90% of carpets and mattresses, HDM alle...