Objective(s):Antibodies against actin, as one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells, are used as internal loading controls in western blot analyses. The aim of this study was to produce polyclonal antibody against a synthetic peptide derived from N-terminal region of β-actin protein to be used as a protein loading control in western ...

introduction: as monoclonal antibodies are potential tools for characterization of soluble or cellular surface antigens, use of these proteins has always been considered in infertility and reproduction research. therefore, in this study, monoclonal antibodies against human sperm surface antigens were produced. material and methods: to produce specific clones against human sperm surface antigens...

in this study we produced and purified a high titer of specific and high affin¬ity fab fragments of anti-digoxin antibody. immunization of rabbits with a conju¬gate of the cardiac glycoside digoxin, coupled by a periodate oxidation method to the amino group of lysine in bovine serum albumin resulted in the production of this type of high titer digoxin-specific antibodies with exceptionally high...

background and objectives: varicella zoster virus (vzv) infection in pregnant women can cause complications for the mother and fetus. the aim of this study was to assess the immunity against vzv among young women before marriage. materials and methods: in this cross-sectional study 250 of women referring to health centers in sanandaj, iran, for pre-marital medical check-up were selected. the vz...

amegakaryocytic thrombocytopenia (amt) is a rare cause of acquired thrombocytopenia. the pathogenesis and treatment of amt is not clearly known. here we demonstrate a 50-year-old man presented with the clinical manifestations of severe thrombocytopenia (7000 platelets/µl) with a marked decrease to absent of megakaryocytes in the bone marrow. the patient did not respond to intravenous immunoglob...

varicella zoster virus (vzv) is a member of herpes family viruses, which causes varicella (chickenpox) after primary infection and herpes zoster (shingles) because of latent virus reactivation from dorsal root ganglia. generally, prevalence of varicella antibodies increases with age. we aimed to compare the prevalence of anti-vzv antibody in children under seven years old, in order to obtain a ...