The generation efficiencies of mutation-induced mice when using engineered zinc-finger nucleases (ZFNs) have been generally 10 to 20% of obtained pups in previous studies. The discovery of high-affinity DNA-binding modules can contribute to the generation of various kinds of novel artificial chromatin-targeting tools, such as zinc-finger acetyltransferases, zinc-finger histone kinases and so on...

Cys2His2 zinc fingers are eukaryotic DNA-binding motifs, capable of distinguishing different DNA sequences, and are suitable for engineering artificial transcription factors. In this work, we used the budding yeast Saccharomyces cerevisiae to study the ability of tailor-made zinc finger proteins to activate the expression of the FLO11 gene, with phenotypic consequences. Two three-finger peptide...

In this study, first, human hand anatomy is investigated dynamically. The tendon configuration of the fingers are also introduced and then modeled to imitate a real human hand index finger in the study because the actuating elements of the fingers are tendons on the phalanges. The prosthetic hand finger model is aimed to replace a real index finger of an amputated human. Therefore, artificial t...

As artificial intelligence (AI) systems and behavior models in military simulations become increasingly complex, it has been difficult for users to understand the activities of computer-controlled entities. Prototype explanation systems have been added to simulators, but designers have not heeded the lessons learned from work in explaining expert system behavior. These new explanation systems a...

Dynamic learning of random stimuli can be described as a random walk among the stable synaptic values. It is shown that prototype extraction can take place in material attractor neural networks when the stimuli are correlated and hierarchically organized. The network learns a set of attractors representing the prototypes in a completely unsupervised fashion and is able to modify its attractors ...

Prototype artificial cell models with designed functional molecules are presented here. Artificial molecular devices based on a giant liposome were prepared to obtain specific properties that cannot be obtained from natural cells. In this context, artificial cell research is seen an extension of “molecular robotics” research. Cooperative and integrated chemical systems will be constructed from ...

Our experiments describe an alternative method of dsDNA recognition using zinc finger (ZF) molecules which bind DNA specifically and with high affinity. Our aim was to develop zinc finger probes which are able to bind to dsDNA molecules at predetermined sites. In our basic approach we used pairs of complementary oligonucleotides to form dsDNAs, containing one of the three SP1-transcription fact...

At present, work on prosthetic hands finds special importance, because this branch of engineering enables the mankind to think for the realizable substitute of human limbs using artificial means. With the advent of realization of various types of micro-motors, this subject of prosthetic hands finds practical realization, because human limbs basically are operated with neuromotors energy being d...

The neural-based control of a robotic hand has many clinical and engineering applications. Current approaches to this problem have been limited due to a lack of understanding of the relationship between neural signals and dynamic finger movements. Here, we present a technique to predict index finger joint angles from neural signals recorded from the associated muscles. The neural signals are co...