prostate-specific antigen (psa) was purified to homogeneity from human seminal plasma by ion-exchange chromatography on a cm-sephadex c-50 and by gel filtration on a sephacryl s-200 column. a single 33-kda protein band appeared in sds-page. high pressure liquid chromatography (hplc) of the purified protein produced a single peak, while isoelectric focusing demonstrated the presence of five diff...

a multiple linear regression model is proposed to calculate capacity factor of  analytes using structural features computed using hyperchem software. the  chemical descriptors of analytes were computed using hyperchem software and  regressed against the experimental capacity factors of analytes collected from the  literature. the absolute average percentage deviation (aard) and individual perce...