Dystonia musculorum (dt) is a mouse inherited sensory neuropathy caused by mutations in the dystonin gene. While the primary pathology lies in the sensory neurons of dt mice, the overt movement disorder suggests motor neurons may also be affected. Here, we report on the contribution of motor neurons to the pathology in dt(27J) mice. Phenotypic dt(27J) mice display reduced alpha motor neuron cel...

The crystal structure of diphtheria toxin (DT) in the absence of nucleotide (nucleotide-free DT) has been determined at 2.3 A resolution to a crystallographic R factor and free R factor of 18.2 and 28.2%, respectively. A comparison of this structure to the previously determined structures of DT in complex with adenyly(3'-5')uridine monophosphate (ApUp) and DT in complex with nicotinamide adenin...

to determine the usefulness of the time intervals obtained from the first derivative of apex cardiogram (da/dt) in assessing contraction and relaxation, 20 hemodynamically and angiographical1y investigated patients with coronary artery disease and 29 patients with hypertensive heart disease were studied. as a control group there were used 50 normal subjects. since contraction and relaxation is ...

The effect of loading rate (dK/dt) on the hydrogen environment-assisted cracking (HEAC) behavior Monel K-500 immersed in 0.6 M NaCl and polarized to −950 −1200 mVSCE was assessed via a slow-rising stress intensity (K) testing framework at dK/dt ranging from 0.2 20 MPa√m/hr. Comparative an inert environment (dry N2 with RH < 5%) 0.1 4 MPa√m/hr revealed clear dK/dt-dependence local plasticity eff...

A new type of displacement transducer for recording the calibrated left apexcardiogram (QLAC) has been evaluated in 69 normal subjects and 99 cardiac patients. Total displacement of QLAC (TD), its peak first derviative (peak dD/dt (t-peak dD/dt). A strong corretation exists between peak dD/dt and TD in normal subjects (r=0.95) and the deviation from the normal relationship allows a separation b...

The concentrations of ortho-tyrosine (o-Tyr) and dityrosine (DT) were measured in noncataractous human lenses in order to assess the role of protein oxidation reactions in the aging of lens proteins. The measurements were conducted by selected ion monitoring-gas chromatography/mass spectrometry using deuterium-labeled internal standards, which provided both high sensitivity and specificity for ...

The role of vacuolar-type H(+)-ATPase (V-ATPase) in the cytotoxic action of diphtheria toxin (DT) was studied by using bafilomycin A1, a specific inhibitor of V-ATPase. Studies with acridine orange showed that the acidification of intracellular acidic compartments was inhibited strongly when Vero cells were treated with 500 nM bafilomycin A1, indicating that bafilomycin effectively inhibits V-A...

Circular dichroism spectroscopy (CD) sensitively reflects even slight alterations of the base stacking geometry in DNA (Tinoco et al. 1980; Johnson et al. 1981). However, the alterations cannot be unambiguously interpreted in conforma­ tional terms so that, for example, the question concerning the origin of the relatively extensive temperature-induced changes in the CD spectrum of a synthetic D...

Left Ventricular Assist Device (LVAD) for Destination Therapy (DT) is an established therapy for end stage heart failure patients who are not transplant candidates. Many DT patients requiring LVADs have had prior open heart surgery, the majority of whom had prior sternotomy. In addition, DT patients tend to be older and more likely to have more significant co-morbidities than their Bridge-To-Tr...

〈μ,D〉 = ∑ i=1 wi 〈ui,uiu T i 〉 Gradient descent Mill maintains the two moments 〈μt,Dt〉 ∈ U as parameter At trial t = 1 . . . T, the Mill 1. Decomposes parameter 〈μt,Dt〉 into a mixture of directions and draws ut from mixture 2. Receives Wind direction xt and gain E [ (ut xt + c) 2] 3. Updates 〈μt,Dt〉 to 〈μ̂t+1, D̂t+1〉 with the gradient of the expected gain on xt μ̂t+1 := μt + 2ηc xt and D̂t+1 := Dt ...