Two serological tests for detection of antibodies to Ehrlichia (previously Cowdria) ruminantium, the causative agent of heartwater, were compared by using field sera collected from sheep and cattle as part of serosurveys in Ghana. Sera selected as either negative or positive by a new polyclonal competitive enzyme-linked immunosorbent assay (PC-ELISA) were tested by the indirect MAP1-B ELISA. Cu...

Enzyme-linked immunosorbent assay (ELISA) and micro-ELISA were evaluated for their ability to detect anti-Fasciola hepatica antibodies in humans by using excretory-secretory antigen. The sensitivity of each method was 100%, but the specificity was 100% for ELISA and 97% for micro-ELISA. The micro-ELISA could be used as a screening assay and ELISA could be used as a confirmatory method for the s...

OBJECTIVE To assess the usefulness of enzyme-linked immunosorbent assay (ELISA) assessment of the combination of bullous pemphigoid antigen 1 (BPAG1) and BPAG2 in the diagnosis of bullous pemphigoid (BP). DESIGN Retrospective study of serum samples from patients with BP. SETTING Tertiary care center. PATIENTS A total of 190 patients with newly diagnosed BP and 78 controls with other autoi...

background and objectives: nasal polyposis is a diseases resulting from complex pathogenetic mechanisms. some studies showed that tgfβ1 had significant role in this pathogenesis. in this study, we investigated the roe of cytokines and mediators in polyp development. material and methods: in this case- control study, healthy nasal mucosal samples were obtained from 24 people undergoing septoplas...

fasciolosis is a disease caused by liver fluck of the genus of fasciola. diagnosis of fasciolosis has been challenging for a long period due to low sensitivity of the coprological diagnostic method. in this study, an in-house dot-elisa method; using excretion–secretory (es ag) and crude (cr ag) antigens of fasciola was described for diagnosis of fasciolosis in cattle. for this purpose, the sera...

conclusions the monitoring of at-risk groups like seasonal migratory farmworkers is necessary to strengthen the healthcare service provided to this population. objectives this study aimed to determine the prevalence of hbv infection and associated risk factors in the reproductive-age female farmworker. materials and methods this cross-sectional study was conducted between january and april 2013...

background and aims: rotaviruses are one of the main causative agents of acute viral gastroenteritis in both young human and animals. several methods have been used for diagnosis of rotavirus infection. most of these methods are not easily available in iran. methods: in our study attempt was made to develop a method which could replace the commercially imported kit. rota virus was purified and ...

background: the aim of this study was to assess the performance of antigen b (agb) isolated from different echinococcus granulosus intermediate hosts and from different cyst locations for immu­nodiagnosis of human cystic echinococcosis (ce). methods: hydatid cyst fluids were collected from lung and liver cysts of sheep, liver cysts of goats, lung cysts of camels and cattle, and liver cysts of h...