Identifying patients with early human immunodeficiency virus infection is an important public health goal. Those individuals who have acquired human immunodeficiency virus infection recently have high viral loads both in their blood and genital secretions, thus facilitating transmission. Identifying and intervening these individuals early in the course of their disease, helps to reduce onward t...

The objective of the work was to evaluate two ELISA’s for detection anti-Map antibodies. 460 serum and fecal samples bovines (n=267), ovines (n=140), goats (n=53) were evaluated. Serums used detecting antibodies using different ELISAs, one in-house ELISA made with low-molecular weight proteins (<100 kD) from antigen ultra-filtrate Map strain 3065, a commercial paratuberculosis diagnost...

I evaluated an enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion (AGID) test simultaneously for the detection of bovine leukemia virus (BLV) antibodies. Total 1,293 serum samples were tested for ELISA and AGID test and the results were compared. The results of ELISA and AGID agreed by 1,156 out of 1,293 (89.4%). All of AGID-positive 356 sera were positive by ELISA. However,...

APPLICATION OF ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS IN RIO GRANDE DO SUL, BRAZIL. The results of an indirect ELISA (I-ELISA) and a competitive ELISA (C-ELISA) for detection of antibody to Brucella abortus in cattle were compared with those of conventional serological tests. The sensitivity of I-ELISA using 230 sera from infected animals was 98.6% and the sp...

An enzyme-linked immunosorbent assay (ELISA) for detection of immunoglobulin G (IgG) and IgM to cardiolipin, lecithin, and cholesterol (VDRL [Venereal Disease Research Laboratory] ELISA) is described. The specificity of the VDRL ELISA for IgG and IgM was 99.6 and 99.5%, respectively, with sera from 1,008 persons without syphilis. For a group of patients with false-positive results in traditiona...

El objetivo del estudio fue comparar las técnicas de inmunofluorescencia indirecta (IFI) y Enzimo inmunoensayo (ELISA) para el diagnóstico Neospora caninum Dubey, Carpenter, Speer, Topper Uggla, 1988 en alpacas la sierra Perú. Se evaluó sensibilidad, especificidad, valor predictivo positivo, negativo concordancia IFI usando referencia un kit comercial ELISA competitivo (cELISA - VMRD). obtuvier...

Paratuberculosis (PTB) is a major disease problem worldwide, and causes major economic losses in the dairy industry. Although PTB has been reported in Korea, no studies have been conducted to determine its prevalence and no program has been developed to control the disease. In this study, the sera of beef (n = 1,056) and dairy cattle (n = 1,105) from all provinces in Korea were tested to determ...

Attachment of poly(ethylene glycol) to proteins can mask immune epitopes to increase serum half-life, reduce immunogenicity, and enhance in vivo biological efficacy. However, PEGylation mediated epitope-masking may also limit sensitivity and accuracy of traditional ELISA. We previously described an anti-PEG-based sandwich ELISA for universal assay of PEGylated molecules. Here, we compared the q...

a total of 65 pollen types were identified from two years atmospheric pollen survey in the environmental conditions of south assam. out of them, eight pollen types viz., acacia auriculiformis, amaranthus spinosus, cassia alata, cleome gynandra, cocos nucifera, imperata cylindrica, ricinus communis and trewia nudiflora, were selected for biochemical studies on the basis of their dominance in the...

b a ckground: west nile virus (wnv) and rift valley fever virus (rvfv) are mosquito-borne viral diseases. the objective of this study was to investigate the rvfv and wnv infections as serologically in different mammalian species (cattle, horse, goat, sheep and water buffalo) in the northern turkey. m e t hods: blood samples randomly collected from 70 each cattle, horse, sheep, goat and water bu...