BACKGROUND Fluorescence activated cell sorting (FACS) is a powerful technique for the qualitative and quantitative detection of biomolecules used widely in both basic research and clinical diagnostic applications. Beads displaying a specific antigen are used to bind antibodies which are then fluorescently labelled using secondary antibodies. As the individual suspension bead passes through the ...

During the thirties, Northrop, Kunitz and their co-workers gave to the world a set of crystallized enzymes and zymogens.1. They also conveyed a set of corresponding ideas: that certain proteolytic enzymes are produced by the body in precursor form, that these precursors can be activated by slight proteolysis, and that this process is usually catalyzed by enzymes. Moreover, they delineated a sys...

Recombinant cytokines are valuable tools for functional studies and candidates for vaccine additives or therapeutic use in various diseases. They can also be used to generate specific antibodies to analyze the roles of different cytokines during immune responses. We generated a mammalian expression system for recombinant cytokines using the equine IgG1 heavy chain constant region as a tag for d...

Two classes of biologic agents can accelerate the production of thrombin from partially purified prothrombin. One class is well represented by platelets, the other by a factor obtainable from the plasma globulins. Representatives of both classes are found not only in blood, but also in crude tissue extracts. These two classes of agents are distinct; and they serve different functions in the clo...

We have designed a novel protein fusion partner (P8CBD) to utilize the co-translational SRP pathway in order to target heterologous proteins to the E. coli inner membrane. SRP-dependence was demonstrated by analyzing the membrane translocation of P8CBD-PhoA fusion proteins in wt and SRP-ffh77 mutant cells. We also demonstrate that the P8CBD N-terminal fusion partner promotes over-expression of ...

Purpose: Study hemodynamic pattern and lipoperoxidation during methylene blue (MB) treatment on taurocholate enterokinase induced acute pancreatitis (AP). Methods: Thirty pigs were equally divided in control group; MB group; AP group; MB previous AP group; and MB after 90 min of induced AP group. MB was given iv in a bolus dose (2mg.kg-1) followed by maintenance dose (2 mg.kg-1.h-1). Hemodynami...